Protein Downstream Processing

Design, Development and Application of High and Low-Resolution Methods

  • Nikolaos E. Labrou

Part of the Methods in Molecular Biology book series (MIMB, volume 1129)

Table of contents

  1. Front Matter
    Pages i-xv
  2. Screening and Design Purification Strategies

    1. Front Matter
      Pages 1-1
    2. Nikolaos E. Labrou
      Pages 3-10
    3. Ignacio Amadeo, Laura Mauro, Eduardo Ortí, Guillermina Forno
      Pages 11-27
    4. Anurag S. Rathore, Rahul Bhambure
      Pages 29-37
    5. Anurag S. Rathore, Sampath Muthukumar
      Pages 39-44
    6. Charlotte Cabanne, Xavier Santarelli
      Pages 45-51
    7. Sunil Kumar, Narayan S. Punekar
      Pages 53-65
  3. Low-Resolution Protein Purification Methods

  4. High-Resolution Protein Purification Methods

    1. Front Matter
      Pages 145-145
    2. Ana Sofia Pina, Íris L. Batalha, Ana Cecília A. Roque
      Pages 147-168
    3. Stefania Deceglie, Claudia Lionetti, Marina Roberti, Palmiro Cantatore, Paola Loguercio Polosa
      Pages 169-180
    4. My Hedhammar, Johan Nilvebrant, Sophia Hober
      Pages 197-204
    5. Johan Nilvebrant, Mikael Åstrand, Sophia Hober
      Pages 205-210
    6. Mark Levisson, Ruud B. Spruijt, Ingrid Nolla Winkel, Servé W. M. Kengen, John van der Oost
      Pages 211-229
    7. Isabel T. Sousa, M. Ângela Taipa
      Pages 231-262
    8. Evangelia Chronopoulou, Nikolaos E. Labrou
      Pages 263-276
    9. Silvia Andrea Camperi, María Camila Martínez-Ceron, Silvana Laura Giudicessi, Mariela Mirta Marani, Fernando Albericio, Osvaldo Cascone
      Pages 277-302
    10. E. G. Vlakh, G. A. Platonova, T. B. Tennikova
      Pages 303-324
    11. Marta Kotasinska, Verena Richter, Marcel Kwiatkowski, Hartmut Schlüter
      Pages 325-338
  5. Assessing Protein Structural Integrity, Purity, and Stabilization

    1. Front Matter
      Pages 339-339
    2. Cosima Damiana Calvano, Cristina De Ceglie, Carlo G. Zambonin
      Pages 365-380
    3. Sona Krizkova, Marketa Ryvolova, Michal Masarik, Ondrej Zitka, Vojtech Adam, Jaromir Hubalek et al.
      Pages 381-396
    4. Anastassios C. Papageorgiou, Jesse Mattsson
      Pages 397-421
    5. Pavel Dráber, Vadym Sulimenko, Tetyana Sulimenko, Eduarda Dráberová
      Pages 443-458
  6. Applications/Case Studies

    1. Front Matter
      Pages 459-459
    2. Karolina Corin, Lotta T. Tegler, Sotirios Koutsopoulos
      Pages 461-486
    3. Pierpaolo Falcicchio, Mark Levisson, Servé W. M. Kengen, Sotirios Koutsopoulos, John van der Oost
      Pages 487-496
    4. Sushrut Arora, B. Vijayalakshmi Ayyar, Richard O’Kennedy
      Pages 497-516
    5. Evangelos Topakas, Paul Christakopoulos
      Pages 517-526
    6. Anna Moosmann, Egbert Müller, Heiner Böttinger
      Pages 527-538
  7. Back Matter
    Pages 547-555

About this book


Proteins are the most diverse group of biologically important substances. With the recent technological advances in the genomics area and the efforts in proteomics research, the rate of discovery for new proteins with unknown structure and function has increased. These proteins generated from genomic approaches present enormous opportunities for research and industrial application. Protein Downstream Processing: Design, Development and Application of High and Low-Resolution Methods is a compilation of chapters within the exciting area of protein purification designed to give the laboratory worker the information needed to design and implement a successful purification strategy. It presents reliable and robust protocols in a concise form, emphasizing the critical aspects on practical problems and questions encountered at the lab bench. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.


Authoritative and easily accessible, Protein Downstream Processing: Design, Development and Application of High and Low-Resolution Methods will be an ideal source of scientific information to advanced students, junior researchers, and scientists involved in health sciences, cellular and molecular biology, biochemistry, and biotechnology and other related areas in both academia and industry.


chromatography electrophoretic techniques protein purification protein recovery recombinant enzymes

Editors and affiliations

  • Nikolaos E. Labrou
    • 1
  1. 1.Laboratory of Enzyme TechnologyAgricultural University of Athens Department of Agr. BiotechnologyAthensGreece

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2014
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-62703-976-5
  • Online ISBN 978-1-62703-977-2
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site