Homing Endonucleases

Methods and Protocols

  • David R. Edgell

Part of the Methods in Molecular Biology book series (MIMB, volume 1123)

Table of contents

  1. Front Matter
    Pages i-x
  2. Mohamed Hafez, Tuhin Kumar Guha, Chen Shen, Jyothi Sethuraman, Georg Hausner
    Pages 37-53
  3. Richard P. Bonocora, Marlene Belfort
    Pages 55-67
  4. Jason M. Wolfs, Benjamin P. Kleinstiver, David R. Edgell
    Pages 97-104
  5. Russell J. Dickson, Gregory B. Gloor
    Pages 223-243
  6. Stefan Pellenz, Raymond J. Monnat Jr.
    Pages 245-264
  7. Back Matter
    Pages 283-284

About this book

Introduction

Homing Endonucleases: Methods and Protocols aims at providing molecular biologists with a comprehensive resource to identify and characterize homing endonucleases from genomic sequence, to deduce the biological basis of binding and cleavage specificity, as well as to provide protocols to redesign endonuclease target specificity for genome-editing applications. Engineering of designer homing endonucleases has set the stage for genome editing of complex eukaryotic genomes with a broad range of potential applications including targeted gene knockouts in model organisms and gene therapy in humans, making this book a valuable resource for future research.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and practical, Homing Endonucleases: Methods and Protocols serves as a key reference for all labs studying site-specific DNA endonucleases.

Keywords

Binding and cleavage specificity Genome editing Mobile genetic elements Recombination-based pathways Site-specific DNA endonucleases

Editors and affiliations

  • David R. Edgell
    • 1
  1. 1.University of Western OntarioLondonCanada

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-62703-968-0
  • Copyright Information Springer Science+Business Media New York 2014
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-62703-967-3
  • Online ISBN 978-1-62703-968-0
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book