Methods and Protocols

  • Juan C. Fontecilla-Camps
  • Yvain Nicolet

Part of the Methods in Molecular Biology book series (MIMB, volume 1122)

Table of contents

  1. Front Matter
    Pages i-x
  2. Perry A. Frey
    Pages 1-3
  3. Carlos Echavarri-Erasun, Simon Arragain, Luis M. Rubio
    Pages 5-18
  4. Carlos Echavarri-Erasun, Simon Arragain, Alessandro A. Scandurra, Luis M. Rubio
    Pages 19-31
  5. Jason C. Crack, Jeffrey Green, Andrew J. Thomson, Nick E. Le Brun
    Pages 33-48
  6. Jon M. Kuchenreuther, Stacey A. Shiigi, James R. Swartz
    Pages 49-72
  7. Oscar Gutiérrez-Sanz, Olaf Rüdiger, Antonio L. De Lacey
    Pages 95-106
  8. Hongxin Wang, Esen Ercan Alp, Yoshitaka Yoda, Stephen P. Cramer
    Pages 125-137
  9. Martin Clémancey, Geneviève Blondin, Jean-Marc Latour, Ricardo Garcia-Serres
    Pages 153-170
  10. Jesse Ward, Emily Ollmann, Evan Maxey, Lydia A. Finney
    Pages 171-187
  11. Luca Bertini, Maurizio Bruschi, Ugo Cosentino, Claudio Greco, Giorgio Moro, Giuseppe Zampella et al.
    Pages 207-268
  12. Back Matter
    Pages 297-299

About this book


Metalloproteins are involved in many key biological processes such as gas transport and metabolism, photosynthesis, cell respiration, the Krebs cycle and many other vital redox reactions. Metalloproteins: Methods and Protocols addresses multiple aspects of metalloenzyme research from the production and purification of metalloproteins using both standard recombinant techniques and a cell-free system, to the electrochemical analysis of metalloproteins, as well as IR techniques, Mössbauer spectroscopy and the theoretical interpretation of metalloenzyme catalytic and redox processes, to name a few topics. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, Metalloproteins: Methods and Protocols seeks to serve both professionals and novices interested in the metalloprotein field.


bioinorganic compounds iron-sulfur proteins metalloenzyme research metalloproteins spectroscopy

Editors and affiliations

  • Juan C. Fontecilla-Camps
    • 1
  • Yvain Nicolet
    • 2
  1. 1.Metalloproteins UnitInstitut de Biologie Structurale (IBS)GrenobleFrance
  2. 2.Metalloproteins UnitInstitut de Biologie Structurale (IBS)GrenobleFrance

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2014
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-62703-793-8
  • Online ISBN 978-1-62703-794-5
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site