Book Volume 1116 2014

DNA Cloning and Assembly Methods

Editors:

ISBN: 978-1-62703-763-1 (Print) 978-1-62703-764-8 (Online)

Table of contents (18 protocols)

  1. Front Matter

    Pages i-xi

  2. No Access

    Protocol

    Pages 1-24

    BioBrick Assembly Standards and Techniques and Associated Software Tools

  3. No Access

    Protocol

    Pages 25-36

    Plasmid Construction by SLIC or Sequence and Ligation-Independent Cloning

  4. No Access

    Protocol

    Pages 37-48

    Quick and Clean Cloning

  5. No Access

    Protocol

    Pages 49-58

    Hierarchical Ligation-Independent Assembly of PCR Fragments

  6. No Access

    Protocol

    Pages 59-72

    USER-Derived Cloning Methods and Their Primer Design

  7. No Access

    Protocol

    Pages 73-87

    Application of the Restriction-Free (RF) Cloning for Multicomponents Assembly

  8. No Access

    Protocol

    Pages 89-101

    A Single-Tube Assembly of DNA Using the Transfer-PCR (TPCR) Platform

  9. No Access

    Protocol

    Pages 103-117

    Circular Polymerase Extension Cloning

  10. No Access

    Protocol

    Pages 119-131

    Golden Gate Cloning

  11. No Access

    Protocol

    Pages 133-151

    Design and Construction of Multigenic Constructs for Plant Biotechnology Using the GoldenBraid Cloning Strategy

  12. No Access

    Protocol

    Pages 153-164

    FX Cloning: A Simple and Robust High-Throughput Cloning Method for Protein Expression

  13. No Access

    Protocol

    Pages 165-181

    Minimum GC-Rich Sequences for Overlap Extension PCR and Primer Annealing

  14. No Access

    Protocol

    Pages 183-192

    Simple Cloning and DNA Assembly in Escherichia coli by Prolonged Overlap Extension PCR

  15. No Access

    Protocol

    Pages 193-208

    Combinatorial Assembly of Clone Libraries Using Site-Specific Recombination

  16. No Access

    Protocol

    Pages 209-234

    Application of In-Fusion™ Cloning for the Parallel Construction of E. coli Expression Vectors

  17. No Access

    Protocol

    Pages 235-244

    Seamless Ligation Cloning Extract (SLiCE) Cloning Method

  18. No Access

    Protocol

    Pages 245-269

    j5 DNA Assembly Design Automation

  19. No Access

    Protocol

    Pages 271-302

    FastPCR Software for PCR, In Silico PCR, and Oligonucleotide Assembly and Analysis

  20. Back Matter

    Pages 303-303