Animal Cell Biotechnology

Methods and Protocols

  • Ralf Pörtner
Part of the Methods in Molecular Biology book series (MIMB, volume 1104)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Cell Line Development and Selection

    1. Front Matter
      Pages 1-1
    2. Wong Cheng Lee, Ali Asgar S. Bhagat, Chwee Teck Lim
      Pages 3-13
    3. João Vidigal, Fabiana Fernandes, Ana S. Coroadinha, Ana P. Teixeira, Paula M. Alves
      Pages 15-27
    4. Henry C. Chiou, Sanjay Vasu, Chao Yan Liu, Isabel Cisneros, Meredith B. Jones, Jonathan F. Zmuda
      Pages 35-55
    5. Glyn N. Stacey, Ed Byrne, J. Ross Hawkins
      Pages 57-73
  3. Techniques for Process Development

    1. Front Matter
      Pages 75-75
    2. Martin Jordan, Matthieu Stettler
      Pages 77-88
    3. Ana Rita Costa, Maria Elisa Rodrigues, Mariana Henriques, Rosário Oliveira, Joana Azeredo
      Pages 105-116
    4. Zhihua Xiao, Michelle Sabourin, Graziella Piras, Stephen F. Gorfien
      Pages 117-135
    5. Maria Elisa Rodrigues, Ana Rita Costa, Mariana Henriques, Joana Azeredo, Rosário Oliveira
      Pages 137-147
  4. Cell Characterisation and Analysis

    1. Front Matter
      Pages 167-167
    2. Michael Butler, Maureen Spearman, Katrin Braasch
      Pages 169-192
    3. Chetan T. Goudar, Richard K. Biener, James M. Piret, Konstantin B. Konstantinov
      Pages 193-209
    4. Judith Wahrheit, Elmar Heinzle
      Pages 211-221
    5. Nelly Aranibar, Michael D. Reily
      Pages 223-236
    6. Tiago M. Duarte, Nuno Carinhas, Ana Carina Silva, Paula M. Alves, Ana P. Teixeira
      Pages 237-247

About this book

Introduction

Animal Cell Biotechnology: Methods and Protocols, Third Edition constitutes a comprehensive manual of state-of-the-art and new techniques for setting up mammalian cell lines for production of biopharmaceuticals, and for optimizing critical parameters for cell culture from lab to final production. The volume is divided into five parts that reflect the processes required for different stages of production. In Part I, basic techniques for establishment of production cell lines are addressed, especially high-throughput synchronization, insect cell lines, transient gene and protein expression, DNA Profiling and Characterisation. Part II addresses tools for process and medium optimization as well as microcarrier technology while Part III covers monitoring of cell growth, viability and apoptosis, metabolic flux estimation, quenching methods as well as NMR-based techniques. Part IV details cultivation techniques, and Part V describes special applications, including vaccine production, baculovirus protein expression, chromatographic techniques for downstream as well as membrane techniques for virus separation. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

 

Animal Cell Biotechnology: Methods and Protocols, Third Edition provides a compendium of techniques for scientists in industrial and research laboratories that use mammalian cells for biotechnology purposes.

Keywords

DNA profiling animal cell lines cell culture gene expression protein expression

Editors and affiliations

  • Ralf Pörtner
    • 1
  1. 1.Bioprozess- und BiosystemtechnikTechnische Universität Hamburg-HarburgHamburgGermany

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-62703-733-4
  • Copyright Information Springer Science+Business Media, LLC 2014
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-62703-732-7
  • Online ISBN 978-1-62703-733-4
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book