Functional Analysis of DNA and Chromatin

  • Juan C. Stockert
  • Jesús Espada
  • Alfonso Blázquez-Castro

Part of the Methods in Molecular Biology book series (MIMB, volume 1094)

Table of contents

  1. Front Matter
    Pages i-xv
  2. Juan C. Stockert, Alfonso Blázquez-Castro, Richard W. Horobin
    Pages 25-38
  3. Maria Luiza S. Mello, Benedicto de Campos Vidal
    Pages 71-78
  4. Benedicto de Campos Vidal, Marina B. Felisbino, Maria Luiza S. Mello
    Pages 99-108
  5. Miguel Pita, Juan Orellana, Paloma Martínez-Rodríguez, Ángel Martínez-Ramírez, Begoña Fernández-Calvín, José L. Bella
    Pages 109-135
  6. Kai J. Neelsen, Arnab Ray Chaudhuri, Cindy Follonier, Raquel Herrador, Massimo Lopes
    Pages 177-208
  7. Colm E. Nestor, James P. Reddington, Mikael Benson, Richard R. Meehan
    Pages 243-258

About this book

Introduction

In Functional Analysis of DNA and Chromatin, expert researchers in the field provide an overview of standard and more recent methods for the functional analysis of the genetic material. These include methods on DNA-dye binding modes, chromatin staining, nuclear dispersion assays, DNA labeling in vivo, sister chromatid exchanges, FISH, DNA and chromatin imaging by fluorescence, electron and atomic force microscopy, detection of apoptotic DNA, cytosine methylation and hydroxy-methylation, DNA thermophoresis, improved methods for histone analysis, chromatin immunoprecipitation, and analysis of rDNA genes and chromatin-associated RNA. Written in the highly successful Methods in Molecular Biology  series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

 Authoritative and practical, Functional Analysis of DNA and Chromatin seeks to aid scientists in the further study of cellular and molecular biology of the nucleus.

Keywords

DNA analysis DNA labeling in vivo DNA thermophoresis DNA-dye binding modes FISH chromatin analysis chromatin imaging by fluorescence chromatin staining cytosine methylation detection of apoptotic DNA electron and atomic force microscopy hydroxy-methylation nuclear dispersion sister chromatid exchanges

Editors and affiliations

  • Juan C. Stockert
    • 1
  • Jesús Espada
    • 2
  • Alfonso Blázquez-Castro
    • 3
  1. 1.Department of BiologyAutonomous University of Madrid Faculty of SciencesMadridSpain
  2. 2.Departamento de Biología, Facultad de CienciasUniversidad Autónoma de MadridMadridSpain
  3. 3.Department of BiologyAutonomous University of Madrid Faculty of SciencesMadridSpain

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-62703-706-8
  • Copyright Information Springer Science+Business Media, New York 2014
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-62703-705-1
  • Online ISBN 978-1-62703-706-8
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book