Brain Development

Methods and Protocols

  • Simon G Sprecher

Part of the Methods in Molecular Biology book series (MIMB, volume 1082)

Table of contents

  1. Front Matter
    Pages i-x
  2. Drosophila Protocols

  3. Other Arthropods

  4. Molluscs

    1. Front Matter
      Pages 115-115
    2. Andreas Wanninger, Tim Wollesen
      Pages 117-125
  5. Xenopus Protocols

    1. Front Matter
      Pages 127-127
    2. Kai-li Liu, Xiu-mei Wang, Zi-long Li, Rong-qiao He, Ying Liu
      Pages 129-141
    3. Cristine Smoczer, Lara Hooker, Saqib S. Sachani, Michael J. Crawford
      Pages 143-154
    4. Jennifer E. Bestman, Hollis T. Cline
      Pages 155-171
  6. Zebrafish Protocols

    1. Front Matter
      Pages 173-173
    2. Adèle Faucherre, Hernán López-Schier
      Pages 187-195
    3. Katherine J. Turner, Thomas G. Bracewell, Thomas A. Hawkins
      Pages 197-214
  7. Chicken Protocols

    1. Front Matter
      Pages 215-215
    2. Andrea Streit, Claudio D. Stern
      Pages 235-251
    3. Irwin Andermatt, Esther T. Stoeckli
      Pages 253-266
  8. Mouse Protocols

    1. Front Matter
      Pages 267-267
    2. Emilie Pacary, François Guillemot
      Pages 285-293
    3. Claudius F. Kratochwil, Filippo M. Rijli
      Pages 295-313
  9. Back Matter
    Pages 315-317

About this book


Providing widely used techniques in genetic model systems and many complementing animal models, Brain Development: Methods and Protocols focuses its expert contributions on two key technical aspects of developmental neurobiology: detection of gene expression and functional characterization of developmental control genes.  Covering animal models such as the fruit fly, zebra fish, chicken, and mouse, this detailed book views in situ hybridization, reporter gene expression, and immunohistochemical staining methods, as well as RNA interference, Morpholino, or transgenic techniques through the prism of these models.  Written in the highly successful Methods in Molecular Biology series format, chapter include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Practical and cutting-edge, Brain Development: Methods and Protocols aims to provide precise technical protocols but also allows for comparing a wide range of protocols in different tissues and species.


Animal models Developmental control genes Developmental neurobiology Drosophila Gene expression detection Genetic model systems Zebrafish

Editors and affiliations

  • Simon G Sprecher
    • 1
  1. 1.Department of BiologyUniversity of FribourgFribourgSwitzerland

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2014
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-62703-654-2
  • Online ISBN 978-1-62703-655-9
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site