Peptide Modifications to Increase Metabolic Stability and Activity

  • Predrag Cudic

Part of the Methods in Molecular Biology book series (MIMB, volume 1081)

Table of contents

  1. Front Matter
    Pages i-ix
  2. Jayati Banerjee, Reena Gyanda, Yi-Pin Chang, Christopher J. Armishaw
    Pages 13-34
  3. Youhong Niu, Yaogang Hu, Haifan Wu, Jianfeng Cai
    Pages 35-46
  4. Back Matter
    Pages 237-241

About this book


In Peptide Modifications to Increase Metabolic Stability and Activity, expert researchers in the field provide summarized methods for preparation, purification of modified peptides, and assessment of their biochemical activities. These methods and protocols include preparation of conformationally constrained peptides, modification of peptide bonds, introduction of nonproteinogenic amino acids, and alteration of peptides’ physical and biological properties by modification of the amino acid side chains and/or terminal residues. With additional chapter that describes new experimental approach for the detection of exogenous peptides within living cells using peptides labeled with heavy isotopes and confocal Raman microscopy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Authoritative and practical, Peptide Modifications to Increase Metabolic Stability and Activity seeks to provide scientists with alternative approaches to peptide modification that many researchers may find applicable to their specific research requirements.


amino acid side chains conformationally constrained peptides exogenous peptides living cells peptide bonds peptide modification peptides metabolic stability peptides’ physical and biological properties synthetic protocols terminal residues

Editors and affiliations

  • Predrag Cudic
    • 1
  1. 1.Torrey Pines Institute for Molecular StuPort St. LucieUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2013
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-62703-651-1
  • Online ISBN 978-1-62703-652-8
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site