Fluorescence Spectroscopy and Microscopy

Methods and Protocols

  • Yves Engelborghs
  • Antonie J.W.G. Visser

Part of the Methods in Molecular Biology book series (MIMB, volume 1076)

Table of contents

  1. Front Matter
    Pages i-xxi
  2. Steady-State Fluorescence Spectroscopy (SSFS)

  3. Time-Resolved Fluorescence Spectroscopy (TRFS)

    1. Front Matter
      Pages 75-75
    2. Carissa M. Vetromile, David M. Jameson
      Pages 77-95
    3. John Paul Eichorst, Kai Wen Teng, Robert M. Clegg
      Pages 97-112
    4. Tomer Orevi, Eitan Lerner, Gil Rahamim, Dan Amir, Elisha Haas
      Pages 113-169
    5. Anatoli V. Digris, Eugene G. Novikov, Victor V. Skakun, Vladimir V. Apanasovich
      Pages 257-277
    6. David J. S. Birch, Philip Yip
      Pages 279-302
    7. Arianna Biesso, Jianhua Xu, Jay R. Knutson
      Pages 303-319
    8. Sergey P. Laptenok, Patrick Nuernberger, Andras Lukacs, Marten H. Vos
      Pages 321-336
    9. Nadtanet Nunthaboot, Kiattisak Lugsanangarm, Arthit Nueangaudom, Somsak Pianwanit, Sirirat Kokpol, Fumio Tanaka
      Pages 337-355
  4. Fluorescent Probe Development (FPD)

    1. Front Matter
      Pages 357-357
    2. Daphne S. Bindels, Joachim Goedhart, Mark A. Hink, Laura van Weeren, Linda Joosen, Theodorus W. J. Gadella Jr.
      Pages 371-417

About this book


Reflecting the expanding field’s need for reliable protocols, Fluorescence Spectroscopy and Microscopy: Methods and Protocols offers techniques from a worldwide team of experts on this versatile and vital subject.  The topics covered fall into four broad categories: steady-state fluorescence spectroscopy, time-resolved fluorescence spectroscopy, fluorescent probe development, and the various sub-categories of fluorescence microscopy, such as fluorescence recovery after photobleaching (FRAP), live cell FRET imaging (FRETim), fluorescence lifetime imaging (FLIM), fluorescence fluctuation spectroscopy (FFS), and single-molecule fluorescence spectroscopy (smFS).  Written as a part of the popular Methods in Molecular Biology series, chapters include the kind of unambiguous detail and key implementation advice that proves essential for successful results.


Comprehensive and practical, Fluorescence Spectroscopy and Microscopy: Methods and Protocols aims to guide both ‘novice’ and established scientists toward furthering their research with these invaluable techniques.


Cuvette-based studies Fluorescence Fluctuation Spectroscopy Fluorescence Lifetime Imaging Fluorescence Recovery After Photobleaching Fluorescent Probe Development Förster Resonance Energy Transfer Imaging Green fluorescent protein (GFP) Single-Molecule Fluorescence Spectroscopy Steady-State Fluorescence Spectroscopy Time-Resolved Fluorescence Spectroscopy

Editors and affiliations

  • Yves Engelborghs
    • 1
  • Antonie J.W.G. Visser
    • 2
  1. 1.KULeuven-Biomolecular Dynamics BioSCENTerLeuvenBelgium
  2. 2.MicroSpectroscopy CentreUniversity of Wageningen BiochemistryWageningenThe Netherlands

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-62703-649-8
  • Copyright Information Springer Science+Business Media, LLC 2014
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-62703-648-1
  • Online ISBN 978-1-62703-649-8
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book