Neuronal Cell Culture

Methods and Protocols

  • Shohreh Amini
  • Martyn K. White

Part of the Methods in Molecular Biology book series (MIMB, volume 1078)

Table of contents

  1. Front Matter
    Pages i-x
  2. Jennifer Gordon, Shohreh Amini, Martyn K. White
    Pages 1-8
  3. Armine Darbinyan, Rafal Kaminski, Martyn K. White, Nune Darbinian, Kamel Khalili
    Pages 45-54
  4. Yonggang Zhang, Wenhui Hu
    Pages 55-63
  5. Joseph F. Bonner, Christopher J. Haas, Itzhak Fischer
    Pages 65-88
  6. S. Ausim Azizi, Barbara Krynska
    Pages 89-96
  7. Kalen R. Dionne, Kenneth L. Tyler
    Pages 97-117
  8. Amanda Parker Struckhoff, Luis Del Valle
    Pages 119-132
  9. Ilker Kudret Sariyer
    Pages 133-139
  10. Hassen S. Wollebo, Baheru Woldemichaele, Martyn K. White
    Pages 141-146
  11. Armine Darbinyan, Paul Pozniak, Nune Darbinian, Martyn K. White, Kamel Khalili
    Pages 147-152
  12. Back Matter
    Pages 163-165

About this book


In Neuronal Cell Culture: Methods and Protocols, the latest aspects of the culture of neural cells are explored by experts in the field who also explain the practical and theoretical considerations of the techniques involved.  Starting with a general overview of the neuronal culturing principles that are described, this detailed volume covers cell line models for neural cells, the isolation and propagation of primary cultures, stem cells, transfection and transduction of neural cultures, and other more advanced techniques.  Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Practical and easy to use, Neuronal Cell Culture: Methods and Protocols will be of interest to scientists at all levels developing cell culture models for neuroscientific studies.


Cell line models Neural cells and tissues Neurological diseases Neuronal culturing Transfection and transduction

Editors and affiliations

  • Shohreh Amini
    • 1
  • Martyn K. White
    • 2
  1. 1.Department of BiologyTemple University College of Science & TechnologyPhiladelphiaUSA
  2. 2.Department of NeuroscienceTemple University School of MedicinePhiladelphiaUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2013
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-62703-639-9
  • Online ISBN 978-1-62703-640-5
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site