Epiblast Stem Cells

Methods and Protocols

  • Ramiro Alberio

Part of the Methods in Molecular Biology book series (MIMB, volume 1074)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Esmail Behboodi, Louise Lam, William G. Gavin, Alla Bondareva, Ina Dobrinski
    Pages 51-67
  3. Ian C. Tobias, Courtney R. Brooks, Jonathan H. Teichroeb, Dean H. Betts
    Pages 69-83
  4. Ivan Vassiliev, Mark B. Nottle
    Pages 85-95
  5. Aida Rodriguez, David A. Contreras, Ramiro Alberio
    Pages 97-110
  6. Shanbo Cao, Fang Wang, Lin Liu
    Pages 111-123
  7. Isabelle Hue, Severine A. Degrelle, Christoph Viebahn
    Pages 125-135
  8. Pauline Aubel, Bertrand Pain
    Pages 137-150
  9. Cantas Alev, Mikiharu Nakano, Yuping Wu, Hiroyuki Horiuchi, Guojun Sheng
    Pages 151-173
  10. Mikkel A. Rasmussen, Vanessa J. Hall, Poul Hyttel
    Pages 185-198
  11. Giovanna Lazzari, Silvia Colleoni, Cesare Galli
    Pages 199-207
  12. Back Matter
    Pages 209-216

About this book


In Epiblast Stem Cells: Methods and Protocols, expect researchers in the field provide a detailed collection of techniques and protocols useful to the study of the biology of the pluripotent epiblast.  These include methods and techniques used to study epiblast development in different amniotes. This collection brings together contributions from the fields of embryology, stem cell biology and developmental biology together, providing a single volume with detailed procedures for the isolation and culture of epiblasts at different stages of development, and techniques for the study of differentiation into specific lineages. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, a complete list of the necessary materials and reagents, detailed laboratory protocols, and extensive notes providing suggestions on troubleshooting and how to overcome common difficulties.


Comprehensive and cutting-edge, Epiblast Stem Cells: Methods and Protocols serves as a resource to individuals interested in studying the biology of pluripotent cells.


Embryo development Epiblast Epiblast stem cells Pluripotency Regenerative biology developmental biology epiblast development pluripotent cells stem cell biology stem cell technologies

Editors and affiliations

  • Ramiro Alberio
    • 1
  1. 1.School of Biosciences Rm 223 South LabUniversity of NottinghamLoughboroughUnited Kingdom

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, New York 2013
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-62703-627-6
  • Online ISBN 978-1-62703-628-3
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site