Pheromone Signaling

Methods and Protocols

  • Kazushige Touhara

Part of the Methods in Molecular Biology book series (MIMB, volume 1068)

Table of contents

  1. Front Matter
    Pages i-xv
  2. Sampling, Chromatographic Purification, and Chemical Analysis of Pheromone Molecules

    1. Front Matter
      Pages 1-1
    2. Tetsu Ando
      Pages 3-14
    3. Jacqueline S. R. Chin, Joanne Y. Yew
      Pages 15-27
    4. Michael Stewart, Cindy F. Baker, Peter W. Sorensen
      Pages 55-69
    5. Xinxing Zhang, Jaime H. Noguez, Yue Zhou, Rebecca A. Butcher
      Pages 71-92
  3. Approaches to Characterize Gene and Function of Pheromone Receptors

  4. Methods in Measuring Neural Responses to Pheromones

  5. Protocols in Analyzing Behavioral and Endocrine Changes Elicited by Pheromones

    1. Front Matter
      Pages 259-259
    2. Daisuke Yamamoto, Soh Kohatsu, Masayuki Koganezawa
      Pages 261-272
    3. Scott J. Neal, Kyuhyung Kim, Piali Sengupta
      Pages 273-283
    4. Peter W. Sorensen
      Pages 293-305
    5. Idan Frumin, Noam Sobel
      Pages 373-394
  6. Back Matter
    Pages 395-399

About this book


Providing experimental methods and protocols for performing pheromone research in a variety of organisms ranging from invertebrates to vertebrates, Pheromone Signaling: Methods and Protocols covers a wide spectrum of experimental approaches necessary for handling pheromone molecules, measuring receptor response and neural activation, and analyzing behavioral output. A great deal of progress has been made in understanding the molecular mechanisms underlying pheromone action, largely due to the discovery of receptor genes, the advancement of imaging techniques, and key multi-disciplinary approaches including aspects of organic chemistry, biochemistry, molecular biology, electrophysiology, and behavioral science. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls.


Authoritative and practical, Pheromone Signaling: Methods and Protocols details the key methodologies utilized in laboratories all over the world, making them accessible to those who want to begin investigation in the area of pheromone research.


Behavioral and endocrine changes Biochemical analysis Chemosensory systems Chromatographic purification Invertebrate and vertebrate models Neural response measurement Pheromone receptors

Editors and affiliations

  • Kazushige Touhara
    • 1
  1. 1.Dept. of Applied Biological ChemistryThe University of Tokyo School of Agricultural & Life SciencesTokyoJapan

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2013
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-62703-618-4
  • Online ISBN 978-1-62703-619-1
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site