Arabidopsis Protocols

  • Jose J. Sanchez-Serrano
  • Julio Salinas

Part of the Methods in Molecular Biology book series (MIMB, volume 1062)

Table of contents

  1. Front Matter
    Pages i-xvi
  2. Growing Arabidopsis

    1. Front Matter
      Pages 1-1
    2. Luz Rivero, Randy Scholl, Nicholas Holomuzki, Deborah Crist, Erich Grotewold, Jelena Brkljacic
      Pages 3-25
    3. Giorgia Batelli, Dong-Ha Oh, Matilde Paino D’Urzo, Francesco Orsini, Maheshi Dassanayake, Jian-Kang Zhu et al.
      Pages 27-51
    4. Bronwyn J. Barkla, Rosario Vera-Estrella, Omar Pantoja
      Pages 53-62
  3. Arabidopsis Resources

    1. Front Matter
      Pages 63-63
    2. Donghui Li, Kate Dreher, Emma Knee, Jelena Brkljacic, Erich Grotewold, Tanya Z. Berardini et al.
      Pages 65-96
    3. Miguel de Lucas, Nicholas J. Provart, Siobhan M. Brady
      Pages 97-136
  4. Genetic Techniques

    1. Front Matter
      Pages 137-137
    2. Johanna A. Molenaar, Joost J. B. Keurentjes
      Pages 139-153
    3. Katherine Bainbridge, Tom Bennett, Peter Crisp, Ottoline Leyser, Colin Turnbull
      Pages 155-163
    4. Silvina Mangano, Cintia Daniela Gonzalez, Silvana Petruccelli
      Pages 165-173
    5. Susan M. Bush, Patrick J. Krysan
      Pages 175-191
    6. Yiping Qi, Colby G. Starker, Feng Zhang, Nicholas J. Baltes, Daniel F. Voytas
      Pages 193-209
    7. Andrew L. Eamens, Marcus McHale, Peter M. Waterhouse
      Pages 211-224
    8. Naoyuki Uchida, Tomoaki Sakamoto, Masao Tasaka, Tetsuya Kurata
      Pages 259-270
    9. Jose M. Alonso, Anna N. Stepanova
      Pages 271-283
    10. Mahmoud W. Yaish, Mingsheng Peng, Steven J. Rothstein
      Pages 285-298
  5. Molecular Biological Techniques

    1. Front Matter
      Pages 299-299
    2. Ryan S. Austin, Steven P. Chatfield, Darrell Desveaux, David S. Guttman
      Pages 301-315
    3. Benjamin B. Minkoff, Heather L. Burch, Michael R. Sussman
      Pages 353-379
    4. Kyonoshin Maruyama, Kazuko Yamaguchi-Shinozaki, Kazuo Shinozaki
      Pages 381-391
    5. Hyunmo Choi, Jun-Young Kim, Young Tae Chang, Hong Gil Nam
      Pages 393-404
    6. Jong-Myong Kim, Taiko Kim To, Maho Tanaka, Takaho A. Endo, Akihiro Matsui, Junko Ishida et al.
      Pages 405-426
  6. Cell Biological Techniques

    1. Front Matter
      Pages 427-427
    2. Sébastien Peter, Klaus Harter, Frank Schleifenbaum
      Pages 429-452
    3. Michael R. Blatt, Christopher Grefen
      Pages 487-507
    4. David W. Galbraith
      Pages 509-537
    5. Daniel von Wangenheim, Gabor Daum, Jan U. Lohmann, Ernst K. Stelzer, Alexis Maizel
      Pages 539-550
    6. Nicolas L. Taylor, Elke Ströher, A. Harvey Millar
      Pages 551-572
  7. Biochemical and Physiological Techniques

    1. Front Matter
      Pages 573-573
    2. Gaetan Glauser, Armelle Vallat, Dirk Balmer
      Pages 597-608
    3. Kirby N. Swatek, Chris B. Lee, Jay J. Thelen
      Pages 609-628
    4. Tino Köster, Dorothee Staiger
      Pages 679-695
    5. José M. Franco-Zorrilla, Roberto Solano
      Pages 697-709
  8. Back Matter
    Pages 711-726

About this book


Arabidopsis Protocols, Third Edition compiles some of the most recent methodologies developed to exploit the Arabidopsis genome. These methodologies cover from the guided access to public resources, to genetic, cell biology, biochemical and physiological techniques, including both those that are widely used as well as those novel techniques likely to open up new avenues of knowledge in the future.  In addition, considering the recent unparalleled progress of the “omics” tools in Arabidopsis, leading experts have contributed sections on genome, transcriptome, proteome, metabolome and other whole-system approaches. Arabidopsis thaliana is acknowledged as the most important plant model system by the scientific community and Arabidopsis research has fundamentally influenced our understanding of the basic biology and ecology of plants. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.


Authoritative and easily accessible, Arabidopsis Protocols, Third Edition seeks to serve both experienced researchers and beginners with its detailed methodologies on this burgeoning scientific field. 


DNA microarray arabidopsis bacterial artificial chromosomes flow cytometry fluorescence microscopy

Editors and affiliations

  • Jose J. Sanchez-Serrano
    • 1
  • Julio Salinas
    • 2
  1. 1.CSIC Centro Nacional de BiotecnologíaMadridSpain
  2. 2.Depto. Biologia de PlantasCSIC Madrid Centro de Investigaciones BiologicasMadridSpain

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2014
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-62703-579-8
  • Online ISBN 978-1-62703-580-4
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site