Metabolomics Tools for Natural Product Discovery

Methods and Protocols

  • Ute Roessner
  • Daniel Anthony Dias

Part of the Methods in Molecular Biology book series (MIMB, volume 1055)

Table of contents

  1. Front Matter
    Pages i-x
  2. Ute Roessner, Daniel Anthony Dias
    Pages 21-28
  3. Nirupama Samanmalie Jayasinghe, Daniel Anthony Dias
    Pages 39-56
  4. Thusitha Wasntha Thilaka Rupasinghe
    Pages 71-80
  5. Lúcia P. Santos Pimenta, Hye Kyong Kim, Robert Verpoorte, Young Hae Choi
    Pages 117-127
  6. Abigail L. Albright, Jonathan M. White
    Pages 149-162
  7. Janice McCauley, Ana Zivanovic, Danielle Skropeta
    Pages 191-205
  8. Rima Caccetta, Hani Al Salami
    Pages 207-218
  9. Elisa J. Hayhoe, Enzo A. Palombo
    Pages 219-225
  10. Ahmed Fares Tawfike, Christina Viegelmann, RuAngelie Edrada-Ebel
    Pages 227-244
  11. Oliver A. H. Jones, Helmut M. Hügel
    Pages 245-266

About this book

Introduction

Classical natural product chemistry is transitioning to modern day metabolomics as a result of the advent of comprehensive analytical platforms and sensitive analytical instrumentation. Therefore, it is worthwhile to summarize recent developments with current analytical platforms and highlight how metabolomics is being integrated into this classical field to dereplicate and profile natural product extracts. Metabolomics Tools for Natural Product Discoveries: Methods and Protocols aims to unite diverse and recently developed methodologies and protocols in order to identify bioactive secondary metabolites for the purpose of drug discovery. Some topics covered in this volume include applications for the extraction of selected natural products from less common sources such as bryophytes and hard corals, various biological assays, comprehensive applications and strategies for GC-MS, LC-MS, and NMR, as well as protocols and strategies for the structure elucidation of isolated natural products.  Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

 

Authoritative and easily accessible Metabolomics Tools for Natural Product Discoveries: Methods and Protocols seeks to serve both professionals and research students with its well-honed methodologies for natural product isolation, biomarker discovery, dereplication, biological assays, and comprehensive metabolomic platforms available for high-throughput analyses.

Keywords

NMR spectroscopy lipidomics mass spectrometry metabolites metabolomics

Editors and affiliations

  • Ute Roessner
    • 1
  • Daniel Anthony Dias
    • 2
  1. 1.ParkvilleAustralia
  2. 2.School of BotanyThe University of MelbourneParkvilleAustralia

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-62703-577-4
  • Copyright Information Springer Science+Business Media, LLC 2013
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-62703-576-7
  • Online ISBN 978-1-62703-577-4
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book