Neural Progenitor Cells

Methods and Protocols

  • Brent A. Reynolds
  • Loic P. Deleyrolle

Part of the Methods in Molecular Biology book series (MIMB, volume 1059)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Culturing Neural Precursor Cells from Primary Tissue

    1. Front Matter
      Pages 1-1
    2. Chiara Cavazzin, Margherita Neri, Angela Gritti
      Pages 25-40
    3. Dhanisha J. Jhaveri, Boris W. Prosper, Perry F. Bartlett
      Pages 41-51
    4. Maurizio Gelati, Daniela Profico, Massimo Projetti-Pensi, Gianmarco Muzi, Giada Sgaravizzi, Angelo Luigi Vescovi
      Pages 65-77
    5. Florian A. Siebzehnrubl, Dennis A. Steindler
      Pages 79-86
    6. Luc Bauchet, Nicolas Lonjon, Florence Vachiery-Lahaye, Alain Boularan, Alain Privat, Jean-Philippe Hugnot
      Pages 87-93
    7. François Féron, Chris Perry, Stéphane D. Girard, Alan Mackay-Sim
      Pages 107-114
  3. Stem Cells vs Progenitor Cells

    1. Front Matter
      Pages 115-115
    2. Sharon A. Louis, Carmen K. H. Mak
      Pages 117-132
  4. Purification of Stem and Progenitor Cells

    1. Front Matter
      Pages 133-133
    2. Geoffrey W. Osborne
      Pages 135-144
  5. Transplantation of Neural Progenitor Cells: Preclinical Models

    1. Front Matter
      Pages 145-145
    2. Tong Zheng, Michael D. Weiss
      Pages 147-156
  6. In Vivo Identification of Neural Precursor Cells

    1. Front Matter
      Pages 181-181
    2. Daniel G. Blackmore, Rodney L. Rietze
      Pages 183-194
    3. Thomas I. H. Park, Henry J. Waldvogel, Johanna M. Montgomery, Edward W. Mee, Peter S. Bergin, Richard L. M. Faull et al.
      Pages 195-225
  7. Clinical Application of Neural Precursor Cell Therapy

    1. Front Matter
      Pages 227-227
    2. Alan Mackay-Sim, François Féron
      Pages 229-237
  8. Back Matter
    Pages 239-241

About this book


The discovery of stem and progenitor cells in the adult mammalian CNS challenged the long standing “no new neuron” doctrine and opened the door to the potential for cell replacement therapy. The process from discovery to clinical applications can be long and tortuous, requiring rigorous steps involving standardized and precise protocols. Neural Progenitor Cells: Methods and Protocols is a collection of practical articles describing techniques used to study neural stem and progenitor cells. The volume also highlights the promise of stem cell-based therapeutic applications for CNS disorders. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.


Authoritative and easily accessible, Neural Progenitor Cells: Methods and Protocols serves both professionals and novices with its well-honed methodologies.


cell cultures neural precursor cell neural progenitor cell progenitor cell stem cell

Editors and affiliations

  • Brent A. Reynolds
    • 1
  • Loic P. Deleyrolle
    • 2
  1. 1.Department of NeurosurgeryUniversity of FloridaGainesvilleUSA
  2. 2.Department of NeurosurgeryUniversity of FloridaGainesvilleUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2013
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-62703-573-6
  • Online ISBN 978-1-62703-574-3
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site