Peptide Nucleic Acids

Methods and Protocols Second Edition

  • Peter E Nielsen
  • Daniel H. Appella

Part of the Methods in Molecular Biology book series (MIMB, volume 1050)

Table of contents

  1. Front Matter
    Pages i-x
  2. Arunava Manna, Srinivas Rapireddy, Raman Bahal, Danith H. Ly
    Pages 1-12
  3. Ethan A. Englund, Ning Zhang, Daniel H. Appella
    Pages 13-18
  4. Roberto Corradini, Tullia Tedeschi, Stefano Sforza, Rosangela Marchelli
    Pages 19-35
  5. Holger Stephan, Christian Foerster, Gilles Gasser
    Pages 37-54
  6. Nicolas Winssinger, Katarzyna Gorska, Mihai Ciobanu, Jean Pierre Daguer, Sofia Barluenga
    Pages 95-110
  7. Irina V. Smolina, Maxim D. Frank-Kamenetskii
    Pages 121-130
  8. Stefano Sforza, Tullia Tedeschi, Mariangela Bencivenni, Alessandro Tonelli, Roberto Corradini, Rosangela Marchelli
    Pages 143-157
  9. Takehiko Shiraishi, Peter E. Nielsen
    Pages 193-205
  10. Shan Goh, Jem Stach, Liam Good
    Pages 223-236
  11. Back Matter
    Pages 237-239

About this book


Exemplifying and illustrating recent exciting advances in PNA chemistry, the second edition of Peptide Nucleic Acids: Methods and Protocols serves as a vital complement to the first edition of the book. Since the discovery of peptide nucleic acids, many interesting new derivatives and analogues in terms of nucleic acid recognition specificity and affinity have emerged. Also, as this detailed volume presents, great ingenuity in exploiting the unique properties of PNAs for a wide variety of applications within drug discovery, medical diagnostics, chemical biology and nanotechnology has unfolded. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.


Peptide Nucleic Acids: Methods and Protocols, Second Edition serves as a source of useful specific methods and protocols as well as a source of inspiration for future developments.


PNA chemistry chemical biology drug discovery medical diagnostics nanotechnology nucleic acid

Editors and affiliations

  • Peter E Nielsen
    • 1
  • Daniel H. Appella
    • 2
  1. 1.Dept. Cellular & Molecular MedicineUniversity of Copenhagen Faculty of Health SciencesCopenhagen NDenmark
  2. 2.NIH NIDDKBethesdaUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, New York 2014
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-62703-552-1
  • Online ISBN 978-1-62703-553-8
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site