Immobilization of Enzymes and Cells

Third Edition

  • Jose M. Guisan

Part of the Methods in Molecular Biology book series (MIMB, volume 1051)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Beatriz Brena, Paula González-Pombo, Francisco Batista-Viera
    Pages 15-31
  3. Fernando López-Gallego, Jose M. Guisán, Lorena Betancor
    Pages 33-41
  4. Fernando López-Gallego, Gloria Fernandez-Lorente, Javier Rocha-Martin, Juan M. Bolivar, Cesar Mateo, Jose M. Guisan
    Pages 59-71
  5. Cesar Mateo, Gloria Fernandez-Lorente, Javier Rocha-Martin, Juan M. Bolivar, Jose M. Guisan
    Pages 73-88
  6. Karen Ovsejevi, Carmen Manta, Francisco Batista-Viera
    Pages 89-116
  7. Veena Singh, Meryam Sardar, Munishwar Nath Gupta
    Pages 129-137
  8. Audrey Sassolas, Akhtar Hayat, Jean-Louis Marty
    Pages 139-148
  9. Ester Polo, Sara Puertas, María Moros, Pilar Batalla, José M. Guisán, Jesús M. de la Fuente et al.
    Pages 149-163
  10. Pablo del Pino, Scott G. Mitchell, Beatriz Pelaz
    Pages 165-207
  11. Akhtar Hayat, Audrey Sassolas, Amina Rhouati, Jean-Louis Marty
    Pages 209-216
  12. Guinevere Strack, Robert Nichols, Plamen Atanassov, Heather R. Luckarift, Glenn R. Johnson
    Pages 217-228
  13. Audrey Sassolas, Akhtar Hayat, Jean-Louis Marty
    Pages 229-239
  14. Jose M. Palomo, Marco Filice, Oscar Romero, Jose M. Guisan
    Pages 255-273
  15. Marta Pastor, Amaia Esquisabel, José Luis Pedraz
    Pages 285-299
  16. Susan R. Sizemore, Robert Nichols, Randi Tatum, Plamen Atanassov, Glenn R. Johnson, Heather R. Luckarift
    Pages 301-312
  17. Pello Sánchez, Rosa María Hernández, José Luis Pedraz, Gorka Orive
    Pages 313-325
  18. Ignacio Moreno-Garrido
    Pages 327-347
  19. Argia Acarregui, Gorka Orive, José Luis Pedraz, Rosa María Hernández
    Pages 349-364
  20. Jorge A. Trelles, Cintia W. Rivero
    Pages 365-374
  21. Back Matter
    Pages 375-377

About this book


For most of industrial applications, enzymes and cells have to be immobilized, via very simple and cost-effective protocols,  in order to be re-used for very long periods of time. From this point of view, immobilization, simplicity and stabilization have to be strongly related concepts. The third edition of Immobilization of Enzymes and Cells expands upon and updates the previous editions with current, detailed protocols for immobilization. With new chapters on protocols for immobilization of enzymes and cells which may be useful to greatly improve the functional properties of enzymes and cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.


Authoritative and practical, Immobilization of Enzymes and Cells, Third Edition demonstrates simple and efficient protocols for the preparation, characterization, and utilization of immobilized enzymes and cells.


biotechnology functional properties of enzymes and cells immobilization immobilized enzymes industrial biocatalysts

Editors and affiliations

  • Jose M. Guisan
    • 1
  1. 1.Instituto de Catlisis y PetroleoquimicaCSICMadridSpain

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, New York 2013
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-62703-549-1
  • Online ISBN 978-1-62703-550-7
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site