Imaging Gene Expression

Methods and Protocols

  • Yaron Shav-Tal

Part of the Methods in Molecular Biology book series (MIMB, volume 1042)

Table of contents

  1. Front Matter
    Pages i-xiii
  2. Imaging Gene Expression and RNA Dynamics in Cells and Organisms

    1. Front Matter
      Pages 1-1
    2. Matthew L. Ferguson, Daniel R. Larson
      Pages 47-60
    3. Tim P. Kaminski, Jan-Hendrik Spille, Claudio Nietzel, Jan Peter Siebrasse, Ulrich Kubitscheck
      Pages 73-85
    4. Alon Kalo, Pinhas Kafri, Yaron Shav-Tal
      Pages 87-99
    5. Jonathan R. Chubb, Michelle Stevense, Danielle Cannon, Tetsuya Muramoto, Adam M. Corrigan
      Pages 101-113
  3. Imaging the Genome and Chromatin Dynamics

    1. Front Matter
      Pages 115-115
    2. Davide Mazza, Sourav Ganguly, James G. McNally
      Pages 117-137
    3. Irena Bronshtein Berger, Eldad Kepten, Yuval Garini
      Pages 139-151
    4. Christian Lanctôt, Peter Meister
      Pages 153-172
    5. Arigela Harikumar, Eran Meshorer
      Pages 173-180
    6. Liron Even-Faitelson, Eden Fussner, Ren Li, Mike Strauss, David P. Bazett-Jones
      Pages 181-195
    7. Nimish Khanna, Qian Bian, Matt Plutz, Andrew S. Belmont
      Pages 197-210
  4. Imaging Nuclear Processes and Sub-nuclear Structures

    1. Front Matter
      Pages 211-211
    2. Marius Reinhart, Corella S. Casas-Delucchi, M. Cristina Cardoso
      Pages 213-225
    3. Niraj M. Shanbhag, Roger A. Greenberg
      Pages 227-235

About this book

Introduction

As imaging technologies and approaches have evolved, the scope of certain imaging techniques has moved far beyond the production of purely illustrative images or appealing time-lapse movies to providing the scientist with a rich range of ways to measure and quantify the biological process and outcome of gene expression.  In Imaging Gene Expression: Methods and Protocols, expert authors offer up-to-date approaches and protocols that scientists in the field have developed, which would benefit the broader scientific community. Divided in three convenient parts, this detailed book covers the output of a gene, namely the RNA molecules that are transcribed from the gene and the way by which these molecules can be tracked or quantified in fixed or living cells, protocols that focus on the gene, DNA, or chromatin, as well as a variety of ways by which nuclear processes intertwined with gene expression can be followed and quantified in living cells as well as approaches for studying several sub-nuclear structures found in eukaryotic cells.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective subjects, lists of materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and up-to-date, Imaging Gene Expression: Methods and Protocols will serve researchers working toward imaging in the context of complete organisms.

Keywords

Chromatin dynamics Eukaryotic cells Gene expression imaging Living cells Nuclear processes RNA molecules Sub-nuclear structures

Editors and affiliations

  • Yaron Shav-Tal
    • 1
  1. 1.Bar-Ilan University Inst. of Nanotech & Advanced MaterialsRamat GanIsrael

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-62703-526-2
  • Copyright Information Springer Science+Business Media, LLC 2013
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-62703-525-5
  • Online ISBN 978-1-62703-526-2
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book