The Inflammasome

Methods and Protocols

  • Christine M. De Nardo
  • Eicke Latz

Part of the Methods in Molecular Biology book series (MIMB, volume 1040)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Mareike Schnaars, Hannes Beckert, Annett Halle
    Pages 1-8
  3. Clara Westwell-Roper, Aisling Dunne, Man Lyang Kim, C. Bruce Verchere, Seth L. Masters
    Pages 9-18
  4. Theo S. Plantinga, Leo A. B. Joosten, Mihai G. Netea
    Pages 29-39
  5. Marie Yang, Claire H. A. Hearnden, Ewa Oleszycka, Ed C. Lavelle
    Pages 41-63
  6. Manira Rayamajhi, Yue Zhang, Edward A. Miao
    Pages 85-90
  7. Andrea Stutz, Gabor L. Horvath, Brian G. Monks, Eicke Latz
    Pages 91-101
  8. Christopher Jakobs, Eva Bartok, Andrej Kubarenko, Franz Bauernfeind, Veit Hornung
    Pages 103-115
  9. Benjamin Faustin, John C. Reed
    Pages 137-152
  10. Jinyao Mo, Joseph A. Duncan
    Pages 153-168
  11. Eran Elinav, Christoph A. Thaiss, Richard A. Flavell
    Pages 185-194
  12. Ryan Grant, Yun-Hee Youm, Anthony Ravussin, Vishwa Deep Dixit
    Pages 195-209
  13. Tamiko Yanagida, Kaoru Orihashi, Hajime Kono
    Pages 211-221
  14. Back Matter
    Pages 223-225

About this book


 Inflammasomes have a seemingly simple architecture. A sensor molecule of the NLR or PYHIN protein family recruits the common adapter molecule apoptosis-associated speck-like protein containing a CARD (ASC). Even so, inflammasome activation is highly regulated at multiple steps and precise details of how inflammasomes are activated at the molecular level remain largely unknown. In The Inflammasome: Methods and Protocols, expert researchers in the field detail methods and protocols to study multiple aspects of inflammasome function. These methods include the generation of inflammasome stimuli, perturbance of cellular homeostasis, assessment of ASC speck formation, monitoring of caspase-1 activity and processing, activation of IL-1β cytokines, and analysis of inflammasome assembly. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.


ATP binding ATPase activity Cells NLRP3 stimulating Amyloid beta adjuvants cellular homeostasis crystalline materials immune system inflammasome stimuli inflammasomes islet amyloid amylin peptides pyroptosis

Editors and affiliations

  • Christine M. De Nardo
    • 1
  • Eicke Latz
    • 2
  1. 1., Institute of Innate ImmunityUniversity of BonnBonnGermany
  2. 2., Institute of Innate ImmunityUniversity of BonnBonnGermany

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, New York 2013
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-62703-522-4
  • Online ISBN 978-1-62703-523-1
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site