Methods and Protocols

  • Bertrand Joseph
  • José Luis Venero

Part of the Methods in Molecular Biology book series (MIMB, volume 1041)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Overview of Microglia Biology

    1. Front Matter
      Pages 1-1
    2. Bertrand Joseph, José Luis Venero
      Pages 3-8
  3. Isolation and Culture of Microglia

  4. Depletion and Transduction of Microglia

    1. Front Matter
      Pages 53-53
    2. Lorena Pont-Lezica, Sabrina Colasse, Alain Bessis
      Pages 55-61
    3. Takahiro Masuda, Makoto Tsuda, Hidetoshi Tozaki-Saitoh, Kazuhide Inoue
      Pages 63-67
  5. Analysis of Microglial Cytokine Production

  6. Analysis of Microglia Activation

    1. Front Matter
      Pages 101-101
    2. Refik Pul, Kandiyil Prajeeth Chittappen, Martin Stangel
      Pages 121-127
    3. Alicia A. Babcock, Martin Wirenfeldt, Bente Finsen
      Pages 129-145
    4. Tom Schilling, Claudia Eder
      Pages 163-182
  7. Analysis of Microglia Polarization

    1. Front Matter
      Pages 183-183
    2. Sadanand M. Gaikwad, Michael T. Heneka
      Pages 185-197
    3. Bryce A. Durafourt, Craig S. Moore, Manon Blain, Jack P. Antel
      Pages 199-211
  8. Co-culture Systems to Analysis Microglia Interactions with Other Cell Types

    1. Front Matter
      Pages 213-213
    2. Fernando G. Correa, Miriam Hernangómez, Carmen Guaza
      Pages 215-229
  9. Analysis of Microglia Functions In Vivo

    1. Front Matter
      Pages 241-241
    2. Beatriz Almolda, Berta González, Bernardo Castellano
      Pages 243-259
    3. Nàdia Villacampa, Beatriz Almolda, Berta González, Bernardo Castellano
      Pages 261-279
    4. Rocío M. de Pablos, Ana M. Espinosa-Oliva, Antonio J. Herrera
      Pages 281-289
    5. Takahiro Masuda, Makoto Tsuda, Hidetoshi Tozaki-Saitoh, Kazuhide Inoue
      Pages 291-294
    6. Ana M. Espinosa-Oliva, Rocío M. de Pablos, Antonio J. Herrera
      Pages 295-305
    7. Jenny A. Garcia, Sandra M. Cardona, Astrid E. Cardona
      Pages 307-317
    8. Satoru Kondo, Shigeo Okabe
      Pages 319-335
  10. Back Matter
    Pages 347-350

About this book


 Key discoveries concerning the different biological functions of microglia in health and disease have attracted scientists from various fields. In Microglia: Methods and Protocols, expert researchers in the field detail methods for selection of the key cellular, molecular and biochemical techniques that are used in studying the many and varied functions of this fascinating cell. These methods and techniques include microglia cell culture for studying microglia activation and functions, as well as their interaction with other cell types both in vitro and in vivo. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.


Authoritative and practical, Microglia: Methods and Protocols is a useful resource for cell biologists, molecular biologists, immunologists, oncologist and neuroscientists.


biological functions both in vitro and in vivo brain development cancer central nervous system dysregulated immune responses infection injury microglia microglia activation and functions microglia cell culture model systems neurodegenerative disease

Editors and affiliations

  • Bertrand Joseph
    • 1
  • José Luis Venero
    • 2
  1. 1., Department of Oncology-PathologyKarolinska InstitutetStockholmSweden
  2. 2., Department of BiochemistryUniversity of SevilleSevilleSpain

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2013
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-62703-519-4
  • Online ISBN 978-1-62703-520-0
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site