Transplantation Immunology

Methods and Protocols

  • Andrea A. Zachary
  • Mary S. Leffell

Part of the Methods in Molecular Biology book series (MIMB, volume 1034)

Table of contents

  1. Front Matter
    Pages i-xiii
  2. Overview Chapters

    1. Front Matter
      Pages 1-1
    2. Sidonia B. G. Eckle, Jamie Rossjohn, James McCluskey
      Pages 3-39
    3. Kathryn J. Wood, Masaaki Zaitsu, Ryoichi Goto
      Pages 71-83
    4. Colin Brinkman, Bryna Burrell, Joseph Scalea, Jonathan S. Bromberg
      Pages 85-101
    5. Gerald Brandacher
      Pages 103-115
    6. Robert A. Montgomery, Mary S. Leffell, Andrea A. Zachary
      Pages 117-125
    7. Jignesh K. Patel, Jon A. Kobashigawa
      Pages 127-143
  3. Methods Chapters

    1. Front Matter
      Pages 145-145
    2. Mike Bunce, Ben Passey
      Pages 147-159
    3. Ana Lazaro, Bin Tu, Ruyan Yang, Yi Xiao, Kanthi Kariyawasam, Jennifer Ng et al.
      Pages 161-195
    4. Elizabeth A. Trachtenberg, Cherie L. Holcomb
      Pages 197-219
    5. Ketevan Gendzekhadze, Laima Gaidulis, David Senitzer
      Pages 221-237
    6. Raja Rajalingam, Elham Ashouri
      Pages 239-255
    7. Jeremy Ryan Peña, Donna Fitzpatrick, Susan L. Saidman
      Pages 257-283
    8. Robert A. Bray
      Pages 285-296
    9. Donna P. Lucas, Mary S. Leffell, Andrea A. Zachary
      Pages 313-318
    10. Annette M. Jackson, Donna P. Lucas, Jessica L. Badders
      Pages 319-329
    11. Adriana Zeevi, John Lunz
      Pages 343-351
    12. Nancy L. Reinsmoen, Chih-Hung Lai
      Pages 353-358
    13. Steven R. Head, Tony Mondala, Terri Gelbart, Phillip Ordoukhanian, Rebecca Chappel, Gilberto Hernandez et al.
      Pages 385-403
  4. Back Matter
    Pages 405-411

About this book


After decades of research in clinical transplantation,  new techniques have been developed that permit a further understanding of the  immune mechanisms underlying immune recognition of allografts and a more accurate and  thorough evaluation of compatibility between donors and recipients. The second edition of Transplantation Immunology: Methods and Protocols expands upon the previous edition with current, detailed methods in transplantation immunology.  The new methods chapters cover four major areas that are being applied in compatibility evaluations and ongoing transplantation immunology research.  Seven overview chapters provide reviews of the molecular basis for alloreactivity, current understanding of humoral and cellular mechanisms,  as well as new developments in thoracic organ transplantation, composite tissue transplantation and in the transplantation of sensitized patients.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.


Authoritative and practical, Transplantation Immunology: Methods and Protocol, Second Edition is devoted to transplantation immunology, both in the practice of compatibility testing and in transplantation research.


HLA antigens HLA specific antibodies KIR systems RNA expression analysis alloreactivity cell based cellular immune function clinical application histocompatibility testing protein array transplantation immunology

Editors and affiliations

  • Andrea A. Zachary
    • 1
  • Mary S. Leffell
    • 2
  1. 1., Immunogenetics LaboratoryJohns Hopkins University School of MedicBaltimoreUSA
  2. 2., Immunogenetics LaboratoryJohns Hopkins University School of MedicBaltimoreUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2013
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-62703-492-0
  • Online ISBN 978-1-62703-493-7
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site