Book Volume 1022 2013

Glycosyltransferases

Methods and Protocols

Editors:

ISBN: 978-1-62703-464-7 (Print) 978-1-62703-465-4 (Online)

Table of contents (31 protocols)

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  1. Front Matter

    Pages i-xvii

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    Protocol

    Pages 1-14

    Glycan Microarray Screening Assay for Glycosyltransferase Specificities

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    Protocol

    Pages 15-28

    A Fluorescence-Based Assay for Core 1 β3Galactosyltransferase (T-Synthase) Activity

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    Protocol

    Pages 29-39

    Structural and Biochemical Analysis of a Bacterial Glycosyltransferase

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    Protocol

    Pages 41-50

    Study of the Biological Functions of Mucin Type Core 3 O-glycans

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    Protocol

    Pages 51-60

    Generation of Anti-sulfated Glycan Antibodies Using Sulfotransferase-Deficient Mice

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    Protocol

    Pages 61-72

    Fluorescent Microscopy as a Tool to Elucidate Dysfunction and Mislocalization of Golgi Glycosyltransferases in COG Complex Depleted Mammalian Cells

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    Protocol

    Pages 73-97

    A Practical Approach to Reconstruct Evolutionary History of Animal Sialyltransferases and Gain Insights into the Sequence–Function Relationships of Golgi-Glycosyltransferases

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    Protocol

    Pages 99-105

    Fluorescent Lectin Staining of Drosophila Embryos and Tissues to Detect the Spatial Distribution of Glycans During Development

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    Protocol

    Pages 107-117

    Photoaffinity Labeling of Protein O-Mannosyltransferases of the PMT1/PMT2 Subfamily

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    Protocol

    Pages 119-128

    Enzymatic Analysis of the Protein O-Glycosyltransferase, Rumi, Acting Toward Epidermal Growth Factor-Like (EGF) Repeats

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    Protocol

    Pages 129-145

    Enzymatic Characterization of Recombinant Enzymes of O-GlcNAc Cycling

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    Protocol

    Pages 147-159

    Antibodies and Activity Measurements for the Detection of O-GlcNAc Transferase and Assay of its Substrate, UDP-GlcNAc

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    Protocol

    Pages 161-171

    In Vitro Glycosylation Assay for Bacterial Oligosaccharyltransferases

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    Protocol

    Pages 173-183

    In Vitro UDP-Sugar:Undecaprenyl-Phosphate Sugar-1-Phosphate Transferase Assay and Product Detection by Thin Layer Chromatography

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    Protocol

    Pages 185-197

    In Vitro O-Antigen Ligase Assay

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    Protocol

    Pages 199-214

    Functional Identification of Bacterial Glucosyltransferase WbdN

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    Protocol

    Pages 215-227

    Methods for the Pasteurella Glycosaminoglycan Synthases: Enzymes that Polymerize Hyaluronan, Chondroitin, or Heparosan Chains

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    Protocol

    Pages 229-247

    Methods for Measuring Class I Membrane-Bound Hyaluronan Synthase Activity

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    Protocol

    Pages 249-275

    Creation and Characterization of Glycosyltransferase Mutants of Trypanosoma brucei

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    Protocol

    Pages 277-282

    Mannose-6-Phosphate: A Regulator of LLO Destruction

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