Methods and Protocols

  • Inka Brockhausen

Part of the Methods in Molecular Biology book series (MIMB, volume 1022)

Table of contents

  1. Front Matter
    Pages i-xvii
  2. Wenjie Peng, Corwin M. Nycholat, Nahid Razi
    Pages 1-14
  3. Fan Zhu, Ren Wu, Hua Zhang, Hui Wu
    Pages 29-39
  4. Tony Lefebvre, Ludivine Drougat, Stephanie Olivier-Van Stichelen, Jean-Claude Michalski, Anne-Sophie Vercoutter-Edouart
    Pages 147-159
  5. Matias A. Musumeci, Maria V. Ielmini, Mario F. Feldman
    Pages 161-171
  6. Xiang Ruan, Miguel A. Valvano
    Pages 185-197
  7. Yin Gao, Anna Vinnikova, Inka Brockhausen
    Pages 199-214
  8. Paul H. Weigel, Amy J. Padgett-McCue, Bruce A. Baggenstoss
    Pages 229-247
  9. Luis Izquierdo, M. Lucia S. Güther, Michael A. J. Ferguson
    Pages 249-275
  10. Ningguo Gao, Mark A. Lehrman
    Pages 277-282
  11. Shinji Takamatsu, Hiroaki Korekane, Kazuaki Ohtsubo, Suguru Oguri, Jong Yi Park, Akio Matsumoto et al.
    Pages 283-298
  12. Maya K. Sethi, Falk F. R. Buettner, Angel Ashikov, Hans Bakker
    Pages 307-320
  13. Shingo Hatakeyama, Toshiaki K. Shibata, Yuki Tobisawa, Chikara Ohyama, Kazuhiro Sugihara, Michiko N. Fukuda
    Pages 369-386
  14. Malene Bech Vester-Christensen, Eric Paul Bennett, Henrik Clausen, Ulla Mandel
    Pages 403-420
  15. Back Matter
    Pages 421-425

About this book


Glycosyltransferases (GTs) are essential for the biosynthesis of complex glycoconjugates and are powerful tools to study the functions of complex glycans in health, development and disease.  Complex glycoconjugates, such as glycoproteins, proteoglycans and glycolipids, are assembled by GTs which synthesize specific linkages between sugars or sugars and protein. This is in contrast to the non-specific or less specific chemical glycation reactions, transglycosylation and  reverse glycosylation reactions. Glycosyltransferases: Methods and Protocols contains a wide range of studies, methods and protocols which form a solid basis for investigations of the role and mechanisms, biology and pathology involving GTs. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.


Authoritative and easily accessible, Glycosyltransferases: Methods and Protocols is a vital contribution to glycobiology and glycopathology, and to applications of these enzymes in biotechnology and drug development. It will prove invaluable to students, postdoctoral fellows, and senior scientists carrying on research of GTs that has been intensified over the last years.


bacterial glycosyltransferases fluorescent microscopy glycan antibodies glycans glycosyltransferases

Editors and affiliations

  • Inka Brockhausen
    • 1
  1. 1.Department of MedicineQueen's UniversityKingstonCanada

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2013
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-62703-464-7
  • Online ISBN 978-1-62703-465-4
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site