Circulating MicroRNAs

Methods and Protocols

  • Nobuyoshi Kosaka

Part of the Methods in Molecular Biology book series (MIMB, volume 1024)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Nobuyoshi Kosaka, Yusuke Yoshioka, Keitaro Hagiwara, Naoomi Tominaga, Takahiro Ochiya
    Pages 1-10
  3. Carolina Villarroya-Beltri, Cristina Gutiérrez-Vázquez, Francisco Sánchez-Madrid, María Mittelbrunn
    Pages 41-51
  4. Frederik J. Verweij, Monique A. J. van Eijndhoven, Jaap Middeldorp, D. Michiel Pegtel
    Pages 53-68
  5. Andrey Turchinovich, Ludmila Weiz, Barbara Burwinkel
    Pages 97-107
  6. Leni Moldovan, Kara Batte, Yijie Wang, Jon Wisler, Melissa Piper
    Pages 129-145
  7. Sota Asaga, Dave S. B. Hoon
    Pages 147-155
  8. Yoshiki Murakami, Toshihito Tanahashi
    Pages 173-182
  9. Alessia Gallo, Ilias Alevizos
    Pages 183-190
  10. Hirohisa Izumi, Nobuyoshi Kosaka, Takashi Shimizu, Kazunori Sekine, Takahiro Ochiya, Mitsunori Takase
    Pages 191-201
  11. Akira Machida, Takuya Ohkubo, Takanori Yokota
    Pages 203-209
  12. Gang Wang, Cheuk Chun Szeto
    Pages 211-220
  13. Luke A. Selth, Scott L. Townley, Joanna L. Gillis, Wayne D. Tilley, Lisa M. Butler
    Pages 235-246
  14. Back Matter
    Pages 269-270

About this book


MicroRNAs (miRNAs) are small non-coding RNAs that regulate various biological phenomena, such as development and homeostasis. The dysregulation of miRNA leads to disease progression, particularly of cancer. In Circulating MicroRNAs: Methods and Protocols,  expert researchers in the field detail recent advances in the isolation, purification and analysis of circulating miRNAs from a variety of sources for research. The book is divided into three main topics. The first section involves the study of secretory miRNAs in cell-cell communication, and the second, the study of circulating miRNAs in body fluids. The last describes the novel techniques used to study circulating miRNAs. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

 Authoritative and practical, Circulating MicroRNAs: Methods and Protocols seeks to aid scientists in dealing with the recent advances of RNAi technology from the bench to the bedside.


cancer cell-cell communication circulating miRNAs exosomes human body fluids miRNAs physiological phenomena purification secretory miRNAs

Editors and affiliations

  • Nobuyoshi Kosaka
    • 1
  1. 1., Div. of Molecular and Cellular MedicineNational Cancer Center Research InstitutTokyoJapan

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2013
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-62703-452-4
  • Online ISBN 978-1-62703-453-1
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site