Neural Development

Methods and Protocols

  • Renping Zhou
  • Lin Mei

Part of the Methods in Molecular Biology book series (MIMB, volume 1018)

Table of contents

  1. Front Matter
    Pages i-xiii
  2. Neuron and Glial Cell Culture

    1. Front Matter
      Pages 1-1
    2. Mi-Yeon Kim, Byoung-San Moon, Kang-Yell Choi
      Pages 3-10
    3. Erik Michael DeBoer, Mladen-Roko Rasin
      Pages 21-37
    4. Yun Peng, Wen-Cheng Xiong, Lin Mei
      Pages 39-47
    5. Tharakeswari Selvakumar, Daniel L. Kilpatrick
      Pages 49-59
    6. Won-Seok Choi, Hyung-Wook Kim, Zhengui Xia
      Pages 61-69
    7. Yangyang Huang, Cheryl F. Dreyfus
      Pages 71-80
    8. Clifton G. Fulmer, Cheryl F. Dreyfus
      Pages 81-91
    9. Yanmei Tao
      Pages 93-104
  3. Gene Expression and Analysis

  4. Axon Pathways and Synapses

    1. Front Matter
      Pages 219-219
    2. Xin Yue, Alexander I. Son, Renping Zhou
      Pages 221-227
    3. Markus Weschenfelder, Franco Weth, Bernd Knöll, Martin Bastmeyer
      Pages 229-246
    4. Eric S. Sweet, Chris L. Langhammer, Melinda K. Kutzing, Bonnie L. Firestein
      Pages 261-268
  5. Morphological Analyses of the Developing Nervous System

    1. Front Matter
      Pages 287-287
    2. Alexander I. Son, Katie Sokolowski, Renping Zhou
      Pages 301-311
    3. Gitanjali Das, Kenneth Reuhl, Renping Zhou
      Pages 313-321
    4. Kengo Funakoshi, Akira Yoshikawa, Yoshitoshi Atobe
      Pages 335-341
  6. Back Matter
    Pages 343-347

About this book


Understanding the molecular and cellular mechanisms underlying the development of specific neural circuits is not just an intellectual curiosity but also central to our ability to develop therapeutic approaches to repair damaged pathways in the future. In Neural Development: Methods and Protocols, experts in the field contribute commonly used protocols to facilitate future research in developmental neuroscience. Split into four convenient sections, this detailed volume covers techniques of culturing neurons and glia as well as their growth and differentiation, methods of gene delivery and down regulation, protocols for analyzing axon growth and guidance plus synapse formation, and, finally, basic methods to analyze brain morphology and axon pathways in developing animals.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Comprehensive and accessible, Neural Development: Methods and Protocols provides key guidance for students and postdoctoral fellows new to developmental neurobiology.


Axon pathways Brain morphology Developmental neuroscience Gene delivery Neuron culturing Synapse formation

Editors and affiliations

  • Renping Zhou
    • 1
  • Lin Mei
    • 2
  1. 1., Department of Chemical BiologyRutgers UniversityPiscatawayUSA
  2. 2., Inst. Molecular Medicine & GeneticsGeorgia Health Sciences UniversityAugustaUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2013
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-62703-443-2
  • Online ISBN 978-1-62703-444-9
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site