Methods and Protocols

  • Federico Innocenti
  • Ron H.N. van Schaik

Part of the Methods in Molecular Biology book series (MIMB, volume 1015)

Table of contents

  1. Front Matter
    Pages i-xiii
  2. Introduction to Pharmacogenomics

    1. Front Matter
      Pages 1-1
  3. Techniques for Interrogating Variation in Human Genes and Genomes

    1. Front Matter
      Pages 23-23
    2. Donna Lee Fackenthal, Pei Xian Chen, Ted Howe, Soma Das
      Pages 25-54
    3. Toshihisa Ishikawa, Yoshihide Hayashizaki
      Pages 55-69
    4. Dirk van den Boom, Matthias Wjst, Robin E. Everts
      Pages 71-85
    5. Cristi R. King, Sharon Marsh
      Pages 97-114
    6. Sandra G. Heil
      Pages 179-188
    7. Tahar van der Straaten, Henk-Jan Guchelaar
      Pages 189-197
  4. Functional Assessment of Genetic Variation: In Vitro and In Vivo Methods

    1. Front Matter
      Pages 199-199
    2. Eric R. Gamazon, R. Stephanie Huang, Nancy J. Cox
      Pages 213-224
    3. Toshihisa Ishikawa, Kanako Wakabayashi-Nakao, Hiroshi Nakagawa
      Pages 225-250
    4. Amber Frick, Oscar Suzuki, Natasha Butz, Emmanuel Chan, Tim Wiltshire
      Pages 263-278
  5. Tools for Translation and Implementation of Pharmacogenetic Markers

    1. Front Matter
      Pages 291-291
    2. Dylan M. Glubb, Steven W. Paugh, Ron H. N. van Schaik, Federico Innocenti
      Pages 293-310
    3. Caroline F. Thorn, Teri E. Klein, Russ B. Altman
      Pages 311-320
    4. Vera H. M. Deneer, Ron H. N. van Schaik
      Pages 345-354
  6. Back Matter
    Pages 355-359

About this book


Understanding an individual's genetic makeup is the key to creating personalized drugs with greater efficacy and safety, and pharmacogenomics aims to study the complex genetic basis of inter-patient variability in response to drug therapy.  Based upon the success of its first edition, the second edition of Pharmacogenomics: Methods And Protocols aims to continue providing readers with high-quality content on the most innovative and commonly adopted technologies in the field of pharmacogenomics as presented by experts in the field.  Broken into several sections, this detailed volume examines techniques for interrogating variation in human genes and genomes, functional assessment of genetic variation, both in vitro and in vivo, as well as tools for translation and implementation of pharmacogenetic markers. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to the respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.


Comprehensive and thoroughly updated, Pharmacogenomics: Methods And Protocols, Second Edition serves as an essential reference and an invaluable source on the latest information in this field.


Drug therapy Individual genetic makeup Personalized medicine Pharmaceutical design Pharmacogenomic technologies

Editors and affiliations

  • Federico Innocenti
    • 1
  • Ron H.N. van Schaik
    • 2
  1. 1.Institute for Pharmacogenomics, and Individualized TherapyUniversity of North CarolinaChapel HillUSA
  2. 2., Dept. of Clinical Chemistry (AKC)Erasmus MCRotterdamNetherlands

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2013
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-62703-434-0
  • Online ISBN 978-1-62703-435-7
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site