Corneal Regenerative Medicine

Methods and Protocols

  • Bernice Wright
  • Che J Connon

Part of the Methods in Molecular Biology book series (MIMB, volume 1014)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Perspectives

    1. Front Matter
      Pages 1-1
    2. Naresh Polisetti, Mohammad Mirazul Islam, May Griffith
      Pages 45-52
  3. Corneal Stem Cell Enrichment and Characterization

  4. Corneal Cell Culture

    1. Front Matter
      Pages 101-101
    2. Tor Paaske Utheim, Torstein Lyberg, Sten Ræder
      Pages 103-129
    3. Naresh Polisetti, Nancy C. Joyce
      Pages 131-139
  5. Development of the Artificial Cornea

    1. Front Matter
      Pages 141-141
    2. Mohammad Mirazul Islam, May Griffith, Kimberley Merrett
      Pages 157-164
    3. Laura J. Bray, Karina A. George, Shuko Suzuki, Traian V. Chirila, Damien G. Harkin
      Pages 165-178
    4. Pallavi Deshpande, Charanya Ramachandran, Virender S. Sangwan, Sheila MacNeil
      Pages 179-185
  6. Back Matter
    Pages 225-230

About this book


Continuous regeneration of the cornea is necessary to maintain this tissue in the transparent state that is essential for vision. Therapy for repair of the damaged anterior cornea is currently addressed through the transplantation of donor corneas or the delivery of limbal epithelial stem cells (LESC) to the ocular surface using amniotic membrane (AM) as a supporting scaffold. Research on the bioengineering of corneal equivalents as replacement tissue is underway to develop viable corneal prosthetics. Corneal Regenerative Medicine: Methods and Protocols provides a concise overview of essential techniques in the field of corneal regenerative medicine, highlighting novel strategies to guide the management of key therapies within this area of medicine. Divided into four convenient sections, topics include the identification, characterisation and cultivation of LESC, as well as the investigation of biopolymers used as the basis for corneal substitutes. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.


Authoritative and easily accessible, Corneal Regenerative Medicine: Methods and Protocols covers the fundamental techniques useful for both the laboratory and clinical settings.  


artificial cornea corneal cell structure corneal stem cell corneal substitute limbal epithelial cell limbal stem cell

Editors and affiliations

  • Bernice Wright
    • 1
  • Che J Connon
    • 2
  1. 1.School of PharmacyUniversity of ReadingReadingUnited Kingdom
  2. 2.School of PharmacyUniversity of ReadingReadingUnited Kingdom

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2013
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-62703-431-9
  • Online ISBN 978-1-62703-432-6
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site