Trinucleotide Repeat Protocols

  • Yoshinori Kohwi
  • Cynthia T. McMurray

Part of the Methods in Molecular Biology book series (MIMB, volume 1010)

Table of contents

  1. Front Matter
    Pages i-xiii
  2. Introduction for Trinucleotide Repeat Diseases

    1. Front Matter
      Pages 1-1
    2. Helen Budworth, Cynthia T. McMurray
      Pages 3-17
    3. Chrystalina A. Antoniades, Colin Watts
      Pages 19-32
  3. Analysis of Synaptic Plasticity

    1. Front Matter
      Pages 33-33
    2. Guang Yang, Feng Pan, Paul C. Chang, Frank Gooden, Wen-Biao Gan
      Pages 35-43
    3. Yi Zuo, Xinzhu Yu, Kelly Tennant, Theresa Jones
      Pages 45-57
  4. Targeting on ES Cells

    1. Front Matter
      Pages 59-59
    2. Chuti Laowtammathron, Anthony W. S. Chan
      Pages 61-77
    3. Geralyn Annett, Gerhard Bauer, Jan A. Nolta
      Pages 79-91
  5. Targeting on RNA

  6. Modification of Proteins and DNAs

    1. Front Matter
      Pages 139-139
    2. Elisabetta Tabolacci, Giovanni Neri
      Pages 141-153
    3. Renate K. Hukema, Ben A. Oostra
      Pages 155-176
    4. Francisca Pérez-Severiano, Sergio Montes, Cristian Gerónimo-Olvera, José Segovia
      Pages 177-200
    5. Sarita Lagalwar, Harry T. Orr
      Pages 201-209
  7. Trinucleotide Repeat Expansion and Detection

    1. Front Matter
      Pages 211-211
    2. Virginia Platt, Do Yup Lee, Christie A. Canaria, Ken Frankel, Susan Bernstein, Cynthia T. McMurray
      Pages 213-230
    3. Judith R. Brouwer, Laurent Foiry, Geneviève Gourdon
      Pages 253-264
    4. Kellie A. Benzow, Michael D. Koob
      Pages 265-276
    5. Kazushi Suzuki, Toshiya Sato, Mitsunori Yamada, Hitoshi Takahashi, Shoji Tsuji
      Pages 277-292
  8. Back Matter
    Pages 293-296

About this book


Two decades have passed since trinucleotide repeat expansion was first discovered in genes responsible for certain neurological diseases. Since then, new technologies have developed and innovative concepts have emerged, which may prove useful in devising therapeutic approaches to neurological diseases. Divided into six convenient sections, Trinucleotide Repeat Protocols, Second Edition covers a wide range of topics such as an overview of trinucleotide repeat diseases, synaptic plasticity, embryonic stem (ES) cell-related protocols with a focus on HD, RNA-related protocols, and analysis of epigenetic modification in fragile X syndrome.  This edition focuses not only on direct analysis of trinucleotide repeat diseases but also on alternative approaches for the analysis of trinucleotide repeat diseases, with the hope that this will result in a better understanding of the mechanisms and future therapeutic prospects for treatment of these diseases. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.


Authoritative and easily accessible, Trinucleotide Repeat Protocols, Second Edition seeks to serve researchers with its thorough methodologies on this expanding field.


DNA modification ES cells RNA protein modification synaptic plasticity trinucleotide repeat disease

Editors and affiliations

  • Yoshinori Kohwi
    • 1
  • Cynthia T. McMurray
    • 2
  1. 1.Life Sciences DivisionLawrence Berkeley National LaboratoryBerkeleyUSA
  2. 2.Life Sciences DivisionLawrence Berkeley National LaboratoryBerkeleyUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2013
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-62703-410-4
  • Online ISBN 978-1-62703-411-1
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site