Plant Lipid Signaling Protocols

  • Teun Munnik
  • Ingo Heilmann

Part of the Methods in Molecular Biology book series (MIMB, volume 1009)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Lipid Analyses

    1. Front Matter
      Pages 1-1
    2. Katharina vom Dorp, Isabel Dombrink, Peter Dörmann
      Pages 43-54
    3. Steven A. Arisz, Teun Munnik
      Pages 63-68
    4. Sunitha Shiva, Hieu Sy Vu, Mary R. Roth, Zhenguo Zhou, Shantan Reddy Marepally, Daya Sagar Nune et al.
      Pages 79-91
    5. Sang-Chul Kim, Lionel Faure, Kent D. Chapman
      Pages 115-127
  3. Enzyme Activities

    1. Front Matter
      Pages 143-143
    2. Yang Ju Im, Irena Brglez, Catherine Dieck, Imara Y. Perera, Wendy F. Boss
      Pages 163-174
    3. Janet L. Donahue, Mustafa Ercetin, Glenda E. Gillaspy
      Pages 175-185
    4. S. M. Teresa Hernández-Sotomayor, J. Armando Muñoz-Sanchez
      Pages 187-192
    5. Přemysl Pejchar, Günther F. E. Scherer, Jan Martinec
      Pages 193-203
    6. Teun Munnik, Ana M. Laxalt
      Pages 219-231
    7. Michael Heinze, Werner Roos
      Pages 241-249
  4. Protein–Lipid Interactions

    1. Front Matter
      Pages 251-251
    2. Teun Munnik, Magdalena Wierzchowiecka
      Pages 253-259
    3. Magdalena M. Julkowska, Johanna M. Rankenberg, Christa Testerink
      Pages 261-271
  5. Imaging

  6. Back Matter
    Pages 303-305

About this book


As scientist begin to understand the complexity of lipid signaling and its roles in plant biology, there is an increasing interest in their analysis. Due to the low abundancy and transient nature of some of these hydrophobic compounds, this is not always easy. In  Plant Lipid Signaling Protocols,  expert researchers in the field detail experimental approaches by which plant signaling lipids can be studied.  These methods and techniques include analysis of plant signaling lipids, including detailed protocols to detect various relevant compounds by targeted or non-targeted approaches; to assay relevant enzyme activities in biological material or using recombinant enzymes; to test for specific binding of signaling lipids to protein partners; or to visualize signaling lipids or lipid-derived signals in living plant cells. Written in the highly successful Methods in Molecular Biology  series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Plant Lipid Signaling Protocols aids plant researchers in the continuing to study the roles of lipid signals.


biological analyses inositol lipids lipid biochemistry lipid kinase lipid phosphatase lipid signaling lipid-derived signals lipidology phosphatidic acid phosphoinositides phospholipase plant cells recombinant enzymes sphingolipids visualizing signaling lipids

Editors and affiliations

  • Teun Munnik
    • 1
  • Ingo Heilmann
    • 2
  1. 1.Swammerdam Inst. Life Science, Sect. Plant PathologyUniversity of AmsterdamAmsterdamNetherlands
  2. 2.Institute for Biochemistry and Biotechno, Department of Cellular BiochemistryMartin-Luther-Univ Halle-WittenbergHalleGermany

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2013
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-62703-400-5
  • Online ISBN 978-1-62703-401-2
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site