Protein-Ligand Interactions

Methods and Applications

  • Mark A. Williams
  • Tina Daviter

Part of the Methods in Molecular Biology book series (MIMB, volume 1008)

Table of contents

  1. Front Matter
    Pages i-x
  2. Introduction and Overview

    1. Front Matter
      Pages 1-1
    2. Mark A. Williams
      Pages 3-34
    3. Tina Daviter, Rémi Fronzes
      Pages 35-62
    4. Peter N. Lowe, Cara K. Vaughan, Tina Daviter
      Pages 63-99
  3. Quantitation of Thermodynamics and Kinetics

    1. Front Matter
      Pages 101-101
    2. Stephen R. Martin, Maria J. Schilstra
      Pages 119-138
    3. Åsa Frostell, Lena Vinterbäck, Hans Sjöbom
      Pages 139-165
  4. Spectroscopic Methods

  5. Ligand Discovery

    1. Front Matter
      Pages 325-325
    2. Geoffrey Holdgate, Stefan Geschwindner, Alex Breeze, Gareth Davies, Nicola Colclough, David Temesi et al.
      Pages 327-355
  6. Molecules in Native Environments

    1. Front Matter
      Pages 415-415
    2. Heiko Keller, Remigiusz Worch, Petra Schwille
      Pages 417-438
    3. Shanica N. Pompey, Peter Michaely, Katherine Luby-Phelps
      Pages 439-453
  7. Structural and Computational Methods

    1. Front Matter
      Pages 455-455
    2. Andrew P. Turnbull, Paul Emsley
      Pages 457-477
    3. Paul J. Gane, A. W. Edith Chan
      Pages 479-499
    4. William R. Pitt, Mark D. Calmiano, Boris Kroeplien, Richard D. Taylor, James P. Turner, Michael A. King
      Pages 501-519
  8. Back Matter
    Pages 521-530

About this book


Proteins are the cell’s workers, their messengers and overseers. In these roles, proteins specifically bind small molecules, nucleic acid and other protein partners. Cellular systems are closely regulated and biologically significant changes in populations of particular protein complexes correspond to very small variations of their thermodynamics or kinetics of reaction. Interfering with the interactions of proteins is the dominant strategy in the development of new pharmaceuticals. Protein Ligand Interactions: Methods and Applications, Second Edition provides a complete introduction to common and emerging procedures for characterizing the interactions of individual proteins. From the initial discovery of natural substrates or potential drug leads, to the detailed quantitative understanding of the mechanism of interaction, all stages of the research process are covered with a focus on those techniques that are, or are anticipated to become, widely accessible and performable with mainstream commercial instrumentation. Written in the highly successful Methods in Molecular Biology series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and accessible, Protein Ligand Interactions: Methods and Applications, Second Edition serves as an ideal guide for researchers new to the field of biophysical characterization of protein interactions – whether they are beginning graduate students or experts in allied areas of molecular cell biology, microbiology, pharmacology, medicinal chemistry or structural biology.


kynetics ligand discovery membrane system spectroscopy thermodynamics

Editors and affiliations

  • Mark A. Williams
    • 1
  • Tina Daviter
    • 2
  1. 1.Birbeck, University of LondonISMB Biophysics Centre, Institute of StrLondonUnited Kingdom
  2. 2.Birkbeck, University of London, School of CrystallographyISMB Biophysics Centre, Institute of StrLondonUnited Kingdom

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2013
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-62703-397-8
  • Online ISBN 978-1-62703-398-5
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site