Methods and Protocols

  • Stella K. Kantartzi

Part of the Methods in Molecular Biology book series (MIMB, volume 1006)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Panagiotis Madesis, Ioannis Ganopoulos, Athanasios Tsaftaris
    Pages 1-13
  3. Discovery and Development of Microsatellites

    1. Front Matter
      Pages 15-15
    2. Valdenice M. Novelli, Mariângela Cristofani-Yaly, Marinês Bastianel, Dario A. Palmieri, Marcos A. Machado
      Pages 17-24
    3. Heng-Sheng Lin, Song-Bin Chang
      Pages 25-55
    4. Elena G. Gonzalez, Rafael Zardoya
      Pages 67-87
  4. Amplification and Visualization

    1. Front Matter
      Pages 131-131
    2. Kundapura V. Ravishankar, Padmakar Bommisetty
      Pages 133-138
    3. Rajarshi Pal, Murali Krishna Mamidi, Anjan Kumar Das, Mahendra Rao, Ramesh Bhonde
      Pages 147-166
  5. Automated Capillary Sequencers

    1. Front Matter
      Pages 179-179
    2. Mark A. Renshaw, Melissa Giresi, J. Orville Adams
      Pages 181-196
    3. Maria del Rosario Herrera, Marc Ghislain
      Pages 197-205
    4. Deborah A. Dean, Phillip A. Wadl, Denita Hadziabdic, Xinwang Wang, Robert N. Trigiano
      Pages 223-243
  6. Scoring and Data Analysis

    1. Front Matter
      Pages 245-245
    2. Pamela Orjuela-Sánchez, Michelle C. Brandi, Marcelo U. Ferreira
      Pages 247-258
    3. M. Humberto Reyes-Valdés
      Pages 259-270
    4. Kyung Seok Kim, Thomas W. Sappington
      Pages 271-295
    5. David A. Lightfoot, Muhammad J. Iqbal
      Pages 297-317
    6. Lluvia Flores-Rentería, Andrew Krohn
      Pages 319-336
  7. Back Matter
    Pages 337-339

About this book


Microsatellites or simple sequence repeats (SSRs) have become the markers of choice for a variety of molecular studies because of their versatility, operational flexibility, and lower cost than other marker systems.  Microsatellites: Methods and Protocols brings together experts in the field to cover this significant area of research.  Broken in to four convenient parts, this volume delves into classical and modern methods for the discovery and development of microsatellite markers, descriptions of amplification and visualization of SSRs, automated capillary sequencers that are widely used for fragment analysis, as well as a variety of methods for the analysis of data obtained by the use of microsatellites. Written for the highly successful Methods in Molecular Biology™ series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Authoritative and invaluable, Microsatellites: Methods and Protocols aims at researchers that need detailed protocols for incorporating microsatellite markers into their projects and expert scientists looking to expand their knowledge of SSRs discovery, use, and analysis.


Capillary sequencers Fragment analysis Genotyping Microsatellite markers Molecular markers SSRs Simple sequence repeats

Editors and affiliations

  • Stella K. Kantartzi
    • 1
  1. 1., Department of Plant, Soil,Southern Illinois University, CarbondaleCarbondaleUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2013
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-62703-388-6
  • Online ISBN 978-1-62703-389-3
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site