Methods and Protocols

  • Kimberly McCall
  • Charles Klein

Part of the Methods in Molecular Biology book series (MIMB, volume 1004)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Analysis of Necrosis In Vitro

    1. Front Matter
      Pages 1-1
    2. Christina Janko, Luis Munoz, Ricardo Chaurio, Christian Maueröder, Christian Berens, Kirsten Lauber et al.
      Pages 3-15
    3. Fredrik Wållberg, Tencho Tenev, Pascal Meier
      Pages 17-29
    4. Isabelle Martins, Oliver Kepp, Laurie Menger, Mickäel Michaud, Sandy Adjemian, Abdul Qader Sukkurwala et al.
      Pages 43-56
  3. Alternative Cell Death Modalities

    1. Front Matter
      Pages 57-57
    2. Qiang Sun, Michael Overholtzer
      Pages 59-66
    3. Lia Danelishvili, Luiz E. Bermudez
      Pages 67-73
  4. Analysis of Cell Death in Mammalian Tissues

    1. Front Matter
      Pages 75-75
    2. S. Burattini, E. Falcieri
      Pages 77-89
    3. Hiroo Yamaguchi, Jie Shen
      Pages 91-113
    4. Xin-Yi Yeap, Shirley Dehn, Jeremy Adelman, Jeremy Lipsitz, Edward B. Thorp
      Pages 115-133
  5. Cell Death in Nonmammalian Model Systems

    1. Front Matter
      Pages 143-143
    2. Xinchen Teng, J. Marie Hardwick
      Pages 161-170
    3. Matt Crook, Avni Upadhyay, Wendy Hanna-Rose
      Pages 171-182
    4. Kai Liu, Yuhong Li, Lei Liu
      Pages 203-213
    5. Allison K. Timmons, Tracy L. Meehan, Tori D. Gartmond, Kimberly McCall
      Pages 215-228
    6. Elena A. Minina, Lada H. Filonova, Victoria Sanchez-Vera, Maria F. Suarez, Geoffrey Daniel, Peter V. Bozhkov
      Pages 229-248
  6. Elena A. Minina, Lada H. Filonova, Victoria Sanchez-Vera, Maria F. Suarez, Geoffrey Daniel, Peter V. Bozhkov
    Pages E1-E1
  7. Back Matter
    Pages 249-252

About this book


Cell death is an essential process in development, and a major contributor to a wide range of human diseases.  Three major classifications of cell death, apoptosis, autophagic cell death and necrosis, have been described for years, and the existence of many more forms of cell death is now accepted. In, Necrosis: Methods and Protocols experts in the field provide a wide range of methods and techniques for the study of necrosis in vitro and in vivo. These include methods and techniques for the analysis of necrosis in mammalian cells, characterization of alternative forms of cell death: entosis and pyroptosis, and analysis of cell death in non-mammalian model sytems and mammalian tissues, including chapters on skin, brain, and heart. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Necrosis: Methods and Protocols describes techniques  in an easy to follow manner, with details so that beginners can succeed with challenging techniques.


Biochemical apoptosis in vivo apoptotic cell death autophagic cell death entosis mammalian cells mammalian tissues necrosis pyroptosis signaling pathways

Editors and affiliations

  • Kimberly McCall
    • 1
  • Charles Klein
    • 2
  1. 1.Department of BiologyBoston UniversityBostonUSA
  2. 2.BrooklineUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2013
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-62703-382-4
  • Online ISBN 978-1-62703-383-1
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site