Ion Channels

Methods and Protocols

  • Nikita Gamper

Part of the Methods in Molecular Biology book series (MIMB, volume 998)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Cloning and Heterologous Expression of Ion Channel Genes

    1. Front Matter
      Pages 1-1
    2. Daunia Laurenti, Lezanne Ooi
      Pages 21-32
    3. Vincent G. L. Postis, Andrea E. Rawlings, Amelia Lesiuk, Stephen A. Baldwin
      Pages 33-54
    4. Hannah M. Kirton, Louisa Pettinger, Nikita Gamper
      Pages 55-64
    5. James Hewinson, Julian F. R. Paton, Sergey Kasparov
      Pages 65-75
  3. Electrophysiological Methods to Study Ion Channel Functions

    1. Front Matter
      Pages 77-77
    2. Bingcai Guan, Xingjuan Chen, Hailin Zhang
      Pages 79-89
    3. Jonathan D. Lippiat, David C. Wrighton
      Pages 91-107
    4. John E. Linley
      Pages 149-157
    5. Jizhe Hao, Jérôme Ruel, Bertrand Coste, Yann Roudaut, Marcel Crest, Patrick Delmas
      Pages 159-170
    6. Carol J. Milligan, Clemens Möller
      Pages 171-187
  4. Imaging and Fluorescence Methods to Study Ion Channels

    1. Front Matter
      Pages 199-199
    2. Sarah Schwarzer, Gregory I. Mashanov, Justin E. Molloy, Andrew Tinker
      Pages 201-208
    3. Alessandro Alaimo, Covadonga Malo, Pilar Areso, Kerman Aloria, Oscar Millet, Alvaro Villarroel
      Pages 217-231
    4. Christopher J. Cockcroft
      Pages 233-241
  5. Biochemical and Structure-Functional Approaches in Ion Channel Studies

  6. Studying Ion Channels in Native Tissues

    1. Front Matter
      Pages 301-301
    2. Kirstin E. Rose, Sylvain Gigout, Nikita Gamper
      Pages 311-320
    3. Elena Mironova, Vladislav Bugay, Oleh Pochynyuk, Alexander Staruschenko, James D. Stockand
      Pages 341-353
    4. Daria V. Ilatovskaya, Alexander Staruschenko
      Pages 355-369
    5. Mykola Mamenko, Oleg Zaika, Roger G. O’Neil, Oleh Pochynyuk
      Pages 371-384
    6. Volodymyr Kucher, Benjamin A. Eaton, James D. Stockand, Nina Boiko
      Pages 385-397
  7. Ion Channels as Research Tools

    1. Front Matter
      Pages 399-399
    2. Stephanie Szobota, Catherine McKenzie, Harald Janovjak
      Pages 417-435
  8. Back Matter
    Pages 437-440

About this book


Every cell in our bodies contains a great variety and number of permeability pathways for various organic and inorganic ions, water, metabolites, nutrients, and signalling molecules. Maintenance and precise control of gating within these pathways are fundamental principles of life as these underlie basic cellular functions such as communication, contractility, and metabolism.  In Ion Channels: Methods and Protocols, Second Edition, experts in the field contribute chapters that focus on the strategies, approaches, methods, and protocols for studying a large family of proteins that form ionic channels in the plasma membrane and intracellular membranes of cells. Using practical examples from the cutting-edge current research, this volume will take a look back at the major methods and approaches that aided the progress toward the current understanding of ion channel function, structural design, and biological roles. The volume also aims to look forward and identify approaches that will lead us to future discoveries. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Authoritative and cutting-edge, Ion Channels: Methods and Protocols, Second Edition will greatly assist researchers searching for specific methodology in studying ion channels on the path toward a greater understanding of these key biological features.


Cell signalling Intracellular membranes Ionic channels Permeability pathways Plasma membrane

Editors and affiliations

  • Nikita Gamper
    • 1
  1. 1.School of Biomedical SciencesUniversity of LeedsLeedsUnited Kingdom

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2013
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-62703-350-3
  • Online ISBN 978-1-62703-351-0
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site