Pluripotent Stem Cells

Methods and Protocols

  • Uma Lakshmipathy
  • Mohan C Vemuri

Part of the Methods in Molecular Biology book series (MIMB, volume 997)

Table of contents

  1. Front Matter
    Pages i-x
  2. Generation of Pluripotent Stem Cells

    1. Front Matter
      Pages 1-1
    2. Hossein Baharvand, Seyedeh-Nafiseh Hassani
      Pages 13-22
    3. Nasir Malik, Mahendra S. Rao
      Pages 23-33
    4. Pauline T. Lieu, Andrew Fontes, Mohan C. Vemuri, Chad C. MacArthur
      Pages 45-56
    5. Alexandria Sams, Mark J. Powers
      Pages 73-89
  3. Culture and Differentiation of Pluripotent Stem Cells

    1. Front Matter
      Pages 91-91
    2. Jasmeet Kaur, Mary Lynn Tilkins
      Pages 93-113
    3. Jasmeet Kaur, Mary Lynn Tilkins, Richard Eckert, Shayne Boucher
      Pages 115-126
    4. Philip Roelandt, Jolien Vanhove, Catherine Verfaillie
      Pages 141-147
  4. Pluripotent Stem Cell Characterization

    1. Front Matter
      Pages 177-177
    2. Rene H. Quintanilla Jr.
      Pages 179-190
    3. Sunali N. Patel, Yalei Wu, Yun Bao, Ricardo Mancebo, Janice Au-Young, Elena Grigorenko
      Pages 191-201
    4. Christopher L. Ricupero, Mavis R. Swerdel, Ronald P. Hart
      Pages 203-216
    5. Ronald V. Abruzzese, Richard A. Fekete
      Pages 217-224
    6. David Ruff, Pauline T. Lieu
      Pages 225-236
  5. Pluripotent Stem Cell Modification and Applications

    1. Front Matter
      Pages 237-237
    2. Catharina Ellerström, Raimund Strehl, Johan Hyllner
      Pages 239-251
    3. Andrew Fontes
      Pages 253-261
  6. Back Matter
    Pages 287-292

About this book


Human pluripotent stem cells such as human embryonic stem cells (hESC) and induced pluripotent stem cells (iPSC) with their unique developmental plasticity hold immense potential as cellular models for drug discovery and in regenerative medicine as a source for cell replacement. While hESC are derived from a developing embryo, iPSC are generated with forced expression of key transcription factors to convert adult somatic cells to ESC-like cells, a process termed reprogramming. Using iPSC overcomes ethical issues concerning the use of developing embryos and it can be generated from patient-specific cells for downstream applications. Pluripotent Stem Cells: Methods and Protocols highlights the best methods and systems for the entire work flow. Divided into four convenient sections, topics include a focus on producing iPSC from diverse somatic sources, media systems for expanding ESC and iPSC with detailed protocols for directed differentiation into specific lineages, commonly used cellular and molecular characterization methods, and the potential application of labeled stem cells with specific methods for cloning, gene delivery and cell engineering.  Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.


Authoritative and easily accessible, Pluripotent Stem Cells: Methods and Protocols seeks to serve both professionals and novices with its well-honed methodologies in an effort to further our knowledge of this essential cellular feature.


cell replacement cellular models drug discovery human embryonic stem cells human pluripotent stem cells stem cell culture stem cell modification

Editors and affiliations

  • Uma Lakshmipathy
    • 1
  • Mohan C Vemuri
    • 2
  1. 1., Primary and Stem Cell SystemsLife TechnologiesCarlsbadUSA
  2. 2., Primary and Stem Cell SystemsLife TechnologiesFrederickUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2013
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-62703-347-3
  • Online ISBN 978-1-62703-348-0
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site