Glycosylation Engineering of Biopharmaceuticals

Methods and Protocols

  • Alain Beck

Part of the Methods in Molecular Biology book series (MIMB, volume 988)

Table of contents

  1. Front Matter
    Pages i-xv
  2. Glyco-Engineering of Therapeutic Proteins

    1. Front Matter
      Pages 1-1
    2. Nassimal El Maï, Sandrine Donadio-Andréi, Chloé Iss, Valérie Calabro, Catherine Ronin
      Pages 19-29
    3. Sylvie Juliant, Marylêne Lévêque, Pierre Cérutti, Annick Ozil, Sylvie Choblet, Marie-Luce Violet et al.
      Pages 59-77
  3. Glycoanalytics

    1. Front Matter
      Pages 79-79
    2. Elsa Wagner-Rousset, Christine Schaeffer-Reiss, Audrey Bednarczyk, Nathalie Corvaïa, Alain Van Dorsselaer, Alain Beck
      Pages 81-91
    3. Marie-Claire Janin-Bussat, Laure Tonini, Céline Huillet, Olivier Colas, Christine Klinguer-Hamour, Nathalie Corvaïa et al.
      Pages 93-113
    4. Arnaud Delobel, Fabrice Cantais, Anicet Catrain, Erell Dereux, Géry Van Vyncht
      Pages 115-143
    5. Diarmuid T. Kenny, Stefan Gaunitz, Catherine A. Hayes, Anki Gustafsson, Magnus Sjöblom, Jan Holgersson et al.
      Pages 145-167
    6. Richard R. Rustandi, Carrie Anderson, Melissa Hamm
      Pages 181-197
    7. Ludovic Landemarre, Eric Duverger
      Pages 221-226
  4. Glycoprotein Complexes Characterization

    1. Front Matter
      Pages 241-241
    2. Cédric Atmanene, Elsa Wagner-Rousset, Nathalie Corvaïa, Alain Van Dorsselaer, Alain Beck, Sarah Sanglier-Cianférani
      Pages 243-268
    3. Victor H. Obungu, Valentina Gelfanova, Lihua Huang
      Pages 291-302
  5. PK/PD Assays for Therapeutic Antibodies

  6. Back Matter
    Pages 353-355

About this book


Glyco-engineering is being developed as a method to control the composition of carbohydrates and to enhance the pharmacological properties of monoclonal antibodies (mAbs) and other proteins.  In Glycosylation Engineering of Biopharmaceuticals: Methods and Protocols, experts in the field provide readers with production and characterization protocols of glycoproteins and glyco-engineered biopharmaceuticals with a focus on mAbs. The volume is divided in four complementary parts dealing with glyco-engineering of therapeutic proteins, glycoanalytics, glycoprotein complexes characterization, and PK/PD assays for therapeutic antibodies.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Authoritative and cutting-edge, Glycosylation Engineering of Biopharmaceuticals: Methods and Protocols serves as an ideal guide for scientists striving to push forward the exciting field of engineered biopharmaceuticals.


Biopharmaceuticals Glyco-engineering Glycoanalytics Glycoproteins Monoclonal antibodies PK/PD assays Therapeutic proteins mAbs

Editors and affiliations

  • Alain Beck
    • 1
  1. 1., Antibody Physico-ChemistryCentre d'Immunologie Pierre Fabre (CIPF)St. Julien-en-GenevoisFrance

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2013
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-62703-326-8
  • Online ISBN 978-1-62703-327-5
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site