Cytochrome P450 Protocols

  • Ian R. Phillips
  • Elizabeth A. Shephard
  • Paul R. Ortiz de Montellano

Part of the Methods in Molecular Biology book series (MIMB, volume 987)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Douglas S. Auld, Henrike Veith, James J. Cali
    Pages 1-9
  3. Kheng B. Lim, Can C. Ozbal, Daniel B. Kassel
    Pages 25-50
  4. Christopher McCullough, Phani Kumar Pullela, Sang-Choul Im, Lucy Waskell, Daniel Sem
    Pages 51-59
  5. Cesar Kenaan, Haoming Zhang, Paul F. Hollenberg
    Pages 61-69
  6. Hugues Ouellet, Eric D. Chow, Shenheng Guan, Jeffery S. Cox, Alma L. Burlingame, Paul R. Ortiz de Montellano
    Pages 79-94
  7. Michael B. Winter, Joshua J. Woodward, Michael A. Marletta
    Pages 95-106
  8. Wesley E. Straub, Clinton R. Nishida, Paul R. Ortiz de Montellano
    Pages 107-113
  9. A. Luthra, M. Gregory, Y. V. Grinkova, I. G. Denisov, S. G. Sligar
    Pages 115-127
  10. Linlin Zhao, Besnik Bajrami, James F. Rusling
    Pages 129-134
  11. M. Teresa Donato, M. José Gómez-Lechón
    Pages 135-148
  12. Kersten S. Rabe, Christof M. Niemeyer
    Pages 149-156
  13. Qian Cheng, F. Peter Guengerich
    Pages 157-162
  14. Rebecca E. Connor, Simona G. Codreanu, Lawrence J. Marnett, Daniel C. Liebler
    Pages 163-176
  15. James B. Y. H. Behrendorff, Wayne A. Johnston, Elizabeth M. J. Gillam
    Pages 177-188
  16. Wayne A. Johnston, Elizabeth M. J. Gillam
    Pages 189-204
  17. Sussan Ghassabian, Michael Murray
    Pages 261-267
  18. Mina Edwards, Lyndsey Houseman, Ian R. Phillips, Elizabeth A. Shephard
    Pages 283-293
  19. Véronique Laurent, Denise Glaise, Tobias Nübel, David Gilot, Anne Corlu, Pascal Loyer
    Pages 295-314
  20. Back Matter
    Pages 315-319

About this book


Cytochromes P450 (CYPs) comprise a large superfamily of proteins that are of central importance in the detoxification or activation of a tremendous number of natural and synthetic hydrophobic xenobiotics, including many therapeutic drugs, chemical carcinogens and environmental pollutants. CYPs are important in mediating interactions between an organism and its chemical environment and in the regulation of physiological processes. Cytochrome P450 Protocols, Third Edition focuses on high-throughput methods for the simultaneous analysis of multiple CYPs, substrates or ligands. Although the emphasis is on CYPs of mammalian origin, it reflects an increasing interest in CYPs of bacterial species. Also included are chapters on cytochrome P450 reductase (the redox partner of CYPs) and the flavin-containing monooxygenases (FMOs), and metabolomic and lipidomic approaches for identification of endogenous substrates of CYPs (‘de-orphanizing’ CYP substrates). Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.


Authoritative and easily accessible, Cytochrome P450 Protocols, Third Edition provides a wide range of techniques accessible to researchers in fields as diverse as biochemistry, molecular biology, pharmacology, toxicology, environmental biology and genetics.


CYP enzymes Cytochrome P450 enzymes DNA shuffling LC-MS mass spectrometry

Editors and affiliations

  • Ian R. Phillips
    • 1
  • Elizabeth A. Shephard
    • 2
  • Paul R. Ortiz de Montellano
    • 3
  1. 1.Sch. of Biological & Chemical SciencesQueen Mary, University of LondonLondonUnited Kingdom
  2. 2., Department of Structural and Molecular BUniversity College LondonLondonUnited Kingdom
  3. 3.University of CaliforniaSan FranciscoUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2013
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-62703-320-6
  • Online ISBN 978-1-62703-321-3
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site