Target Identification and Validation in Drug Discovery

Methods and Protocols

  • Jurgen Moll
  • Riccardo Colombo

Part of the Methods in Molecular Biology book series (MIMB, volume 986)

Table of contents

  1. Front Matter
    Pages i-xv
  2. Background

    1. Front Matter
      Pages 1-1
    2. Marta Cortés-Cros, Tobias Schmelzle, Volker M. Stucke, Francesco Hofmann
      Pages 3-13
  3. DNA as a Tool to Modulate Drug Targets

  4. RNA Interference: From Design to Data Analysis

    1. Front Matter
      Pages 57-57
    2. Sebastian Petri, Gunter Meister
      Pages 59-71
    3. Wanling Liang, A. James Mason, Jenny K. W. Lam
      Pages 73-87
    4. Meredith C. Henderson, David O. Azorsa
      Pages 89-95
    5. Konstantinos Drosopoulos, Spiros Linardopoulos
      Pages 97-104
    6. Martin Stöter, Antje Niederlein, Rico Barsacchi, Felix Meyenhofer, Holger Brandl, Marc Bickle
      Pages 105-122
  5. Protein-Focused Technologies

    1. Front Matter
      Pages 139-139
    2. Jean-Yves Trosset, Nicolas Vodovar
      Pages 141-164
    3. Janos Roszik, Gábor Tóth, János Szöllősi, György Vereb
      Pages 165-178
    4. Maria Isabella Sereni, Mariaelena Pierobon, Roberto Angioli, Emanuel F. Petricoin III, Mitchell J. Frederick
      Pages 187-214
  6. Innovative Cell Culture Techniques to Mimic Tissue Microenvironments

    1. Front Matter
      Pages 215-215
    2. Miriam Zimmermann, Carol Box, Suzanne A. Eccles
      Pages 227-252
    3. Maria Vinci, Carol Box, Miriam Zimmermann, Suzanne A. Eccles
      Pages 253-266
  7. Genetically Engineered Animal Models to Study Gene Functions

    1. Front Matter
      Pages 279-279
    2. Aljoscha Kleinhammer, Wolfgang Wurst, Ralf Kühn
      Pages 307-323
  8. Translational Methods to Validate Biomarkers

  9. Back Matter
    Pages 367-371

About this book


The major reason for the elevated costs of drug development in the pharmaceutical industry is the high attrition rate. In Target Identification and Validation in Drug Discovery: Methods and Protocols, expert researchers in the field detail many of the methods which are now commonly used to identify and validate new target. These include methods and approaches covering biochemical, cell based, in vivo models and translational methods. Chapters also include selected case reports that demonstrate the integration of these technologies to real life experiences and to demonstrate the multiple use of more than one technology to increase knowledge on a specific target. These Written in the highly successful Methods in Molecular Biology™ series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results in the laboratory.


Thorough and intuitive, Target Identification and Validation in Drug Discovery: Methods and Protocols contains a comprehensive list of essential methods and clear protocols to follow.


Omics Oncology biochemical cell based cell culture technologies drug development in vivo models in-silico translational methods

Editors and affiliations

  • Jurgen Moll
    • 1
  • Riccardo Colombo
    • 2
  1. 1.Director, Department of PharmacologyBoehringer Ingelheim RCV GmbH & Co KGViennaAustria
  2. 2.Project and Team Leader, Cell BiologyNerviano Medical SciencesNervianoItaly

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2013
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-62703-310-7
  • Online ISBN 978-1-62703-311-4
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site