Tissue-Protective Cytokines

Methods and Protocols

  • Pietro Ghezzi
  • Anthony Cerami

Part of the Methods in Molecular Biology book series (MIMB, volume 982)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Michael Brines, Anthony Cerami
    Pages 1-11
  3. Omolara O. Ogunshola, Anna Yu. Bogdanova
    Pages 13-41
  4. Pietro Ghezzi, Darrell Conklin
    Pages 43-58
  5. Eithan Galun, Stefan Rose-John
    Pages 59-77
  6. Annelise Letourneur, Edwige Petit, Simon Roussel, Omar Touzani, Myriam Bernaudin
    Pages 79-101
  7. Zübeyde Erbayraktar, Necati Gökmen, Osman Yılmaz, Serhat Erbayraktar
    Pages 103-112
  8. Christopher M. Traudt, Sandra E. Juul
    Pages 113-126
  9. Catharine H. Duman, Samuel S. Newton
    Pages 127-140
  10. Samson Kumar Gaddam, Jovany Cruz, Claudia Robertson
    Pages 141-162
  11. Bianchi Roberto, Lombardi Raffaella, Porretta-Serapiglia Carla, Lauria Giuseppe
    Pages 197-211
  12. Andrew Dilley
    Pages 213-225
  13. Domenico Ribatti
    Pages 227-235
  14. Mark I. Talan, Roberto Latini
    Pages 265-302
  15. Anne-Lise Bienvenu, Stephane Picot
    Pages 315-324
  16. Back Matter
    Pages 325-328

About this book


While many cytokines are known for their inflammatory action, there is a growing interest in the tissue-protective effects of some cytokines. The prototypic tissue-protective cytokine is EPO. Initially described as neuro-protective, it is beneficial in animal models of ischemic and other types of injury. Scientists had to overcome the notion that EPO had only erythropoietic actions, was only produced by the kidney, and that its receptor was only present in erythroid progenitor cells. The use of in vitro and in vivo disease models was essential to demonstrate the protective effects of EPO. Reproducible models will be needed for the further study of the mechanism of action of EPO and for the identification of other tissue-protective cytokines.

 In Tissue-Protective Cytokines: Methods and Protocols, expert researchers in the field detail the key models that have been used to characterize the tissue-protective actions of cytokines. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, provide step-by-step laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

 Thorough and intuitive, Tissue Protective Cytokines: Methods and Protocols aids scientists in continuing to study tissue-protection that will be a new field of interest of cytokine biology, both in discovering novel actions of known cytokines and in developing new drugs.


EPO cytokine biology erythroid progenitor cells erythropoietic cytokine neuroprotective non-erythropoietic EPO platelets

Editors and affiliations

  • Pietro Ghezzi
    • 1
  • Anthony Cerami
    • 2
  1. 1.Medical SchoolBrighton and SussexFalmerUnited Kingdom
  2. 2.Leiden University Medical CenterLeidenNetherlands

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2013
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-62703-307-7
  • Online ISBN 978-1-62703-308-4
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site