Protein Acetylation

Methods and Protocols

  • Sandra B. Hake
  • Christian J. Janzen

Part of the Methods in Molecular Biology book series (MIMB, volume 981)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Barry M. Zee, Benjamin A. Garcia
    Pages 1-11
  3. Caroline A. Evans, Saw Yen Ow, Duncan L. Smith, Bernard M. Corfe, Phillip C. Wright
    Pages 13-23
  4. Caroline A. Evans, John R. Griffiths, Richard D. Unwin, Anthony D. Whetton, Bernard M. Corfe
    Pages 25-36
  5. Bernard M. Corfe, Josephine Kilner, Joanna Chowdry, Roderick S. P. Benson, Gareth J. Griffiths, Caroline A. Evans
    Pages 37-45
  6. Chandan Mandal, Chitra Mandal
    Pages 57-93
  7. Rune H. Evjenth, Petra Van Damme, Kris Gevaert, Thomas Arnesen
    Pages 95-102
  8. Petra Van Damme, Thomas Arnesen, Bart Ruttens, Kris Gevaert
    Pages 115-126
  9. Jin-ying Lu, Yu-yi Lin, Jef D. Boeke, Heng Zhu
    Pages 151-165
  10. John C. Shimko, Cecil J. Howard, Michael G. Poirier, Jennifer J. Ottesen
    Pages 177-192
  11. Matthew Johnson, Michael A. Geeves, Daniel P. Mulvihill
    Pages 193-200
  12. Alexander-Thomas Hauser, Julia M. Gajer (née Wagner), Manfred Jung
    Pages 211-227
  13. Tielong Gao, Chao Yang, Yujun George Zheng
    Pages 229-238
  14. Mohammed Arif, Dhanasekaran Karthigeyan, Soumik Siddhanta, G. V. Pavan Kumar, Chandrabhas Narayana, Tapas K. Kundu
    Pages 239-261
  15. Back Matter
    Pages 263-266

About this book


Thousands of proteins have been identified to be acetylated. Immense research power has been dedicated to experiments to solve the biological implications of each and every protein acetylation. Two particular sites of protein acetylation have been described intensively: the N-terminal methionine residue of a nascent protein and lysine residues within a protein. In Protein Acetylation: Methods and Protocols, expert researchers in the field detail many of the methods which are now commonly used to study protein acetylation. These include methods and techniques for identification of protein acetylation, column- and gel electrophoresis-based approaches, computationally prediction,  and the biological response to protein acetylation. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.


Authoritative and practical, Protein Acetylation: Methods and Protocols seeks to aid scientists in the further study of the technical aspects involved in understanding protein acetylation.


N-terminal methionine residue acetylated column-electrophoresis-based computationally predict gel electrophoresis-based lysine residues mass spectrometry nascent protein protein acetylation

Editors and affiliations

  • Sandra B. Hake
    • 1
  • Christian J. Janzen
    • 2
  1. 1.Adolf-Butenandt-Institut, Department of Molecular BiologyLudwig-Maximilians-Universität MünchenMünchenGermany
  2. 2., Dep. of Cell & Developmental BiologyBiocenter University of WürzburgWürzburgGermany

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2013
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-62703-304-6
  • Online ISBN 978-1-62703-305-3
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site