Dictyostelium discoideum Protocols

  • Ludwig Eichinger
  • Francisco Rivero

Part of the Methods in Molecular Biology book series (MIMB, volume 983)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Christina Schilde, Pauline Schaap
    Pages 1-15
  3. Salvatore Bozzaro
    Pages 17-37
  4. William F. Loomis
    Pages 39-58
  5. Petra Fey, Robert J. Dodson, Siddhartha Basu, Rex L. Chisholm
    Pages 59-92
  6. Annette Müller-Taubenberger, Hellen C. Ishikawa-Ankerhold
    Pages 93-112
  7. Tracy E. Douglas, Debra A. Brock, Boahemaa Adu-Oppong, David C. Queller, Joan E. Strassmann
    Pages 113-124
  8. Jimmie Hällman, Lotta Avesson, Johan Reimegård, Max Käller, Fredrik Söderbom
    Pages 125-138
  9. Edward Roshan Miranda, Gregor Rot, Marko Toplak, Balaji Santhanam, Tomaz Curk, Gad Shaulsky et al.
    Pages 139-171
  10. James L. Platt, Nick A. Kent, Adrian J. Harwood, Alan R. Kimmel
    Pages 173-183
  11. Christa L. Feasley, Alba Hykollari, Katharina Paschinger, Iain B. H. Wilson, Christopher M. West
    Pages 205-229
  12. Neil J. Buttery, Jeff Smith, David C. Queller, Joan E. Strassmann
    Pages 231-248
  13. Jan Faix, Joern Linkner, Benjamin Nordholz, James L. Platt, Xin-Hua Liao, Alan R. Kimmel
    Pages 249-267
  14. Douwe M. Veltman, Peter J. M. Van Haastert
    Pages 269-281
  15. Petros Batsios, Otto Baumann, Ralph Gräf, Irene Meyer
    Pages 283-294
  16. Anne-Marie C. Couto, Nicholas D. Lakin, Catherine J. Pears
    Pages 295-310
  17. Patrick Hofmann, Janis Kruse, Christian Hammann
    Pages 311-323
  18. Jessica E. Accari, Sam Manna, Paul R. Fisher, Christian Barth
    Pages 325-344
  19. Sarah J. Annesley, Sergio Carilla-Latorre, Ricardo Escalante, Paul R. Fisher
    Pages 345-366
  20. Natascha Sattler, Roger Monroy, Thierry Soldati
    Pages 383-402
  21. Sonia Arafah, Sébastien Kicka, Valentin Trofimov, Monica Hagedorn, Nuria Andreu, Siouxsie Wiles et al.
    Pages 403-417
  22. Olga Shevchuk, Michael Steinert
    Pages 419-429
  23. Ivo Finsel, Christine Hoffmann, Hubert Hilbi
    Pages 431-443
  24. Ana Mesquita, Javier Calvo-Garrido, Sergio Carilla-Latorre, Ricardo Escalante
    Pages 461-470
  25. Back Matter
    Pages 471-479

About this book


Dictyostelium discoideum is a well-establish eukaryotic model organism that offers unique advantages for studying fundamental cellular processes, including signal transduction, random and directed cell motility, cytokinesis, endocytosis and vesicle transport and development. Dictyostelium is also increasingly used for the investigation of human disease genes and the crosstalk between host and pathogen. The availability of the genome sequence at a mouse click together with a whole range of supporting information and resources along with a powerful armoury of molecular genetics techniques have considerably enhanced the experimental attractiveness of D. discoideum in recent years.


The second edition of Dictyostelium discoideum Protocols incorporates the most recent developments in a number of fields. The book is divided into four parts. The first part provides an introduction to the amoebozoa and community resources. The second part presents large-scale analysis methods made possible by the completion of the Dictyostelium genome sequence. The third part is dedicated to molecular genetics techniques, cell biological, biochemical and biophysical methods. The chapters in the fourth part describe the use of Dictyostelium as a model system to study vesicle formation, trafficking and infection by bacterial pathogens.


Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

 Authoritative and practical, Dictyostelium discoideum Protocols, Second Edition seeks to aid scientists in the application of innovating techniques to study a range of fundamental biological processes in this attractive model organism.


Dictyostelium amoebozoa bacterial pathogens biochemical methods biophysical methods cell biological genome-wide analyses molecular genetic techniques vesicle formation

Editors and affiliations

  • Ludwig Eichinger
    • 1
  • Francisco Rivero
    • 2
  1. 1., Centre for BiochemistryUniversity of CologneKölnGermany
  2. 2., Centre for Biomedical ResearchUniversity of HullHullMontserrat

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2013
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-62703-301-5
  • Online ISBN 978-1-62703-302-2
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site