Enzyme Engineering

Methods and Protocols

  • James C. Samuelson

Part of the Methods in Molecular Biology book series (MIMB, volume 978)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Neil A. Demarse, Marie C. Killian, Lee D. Hansen, Colette F. Quinn
    Pages 21-30
  3. Olga Paley, Giulia Agnello, Jason Cantor, Tae Hyun Yoo, George Georgiou, Everett Stone
    Pages 31-44
  4. Sarah K. Baxter, Abigail R. Lambert, Andrew M. Scharenberg, Jordan Jarjour
    Pages 45-61
  5. Ting Li, Bing Yang
    Pages 63-72
  6. Misha V. Golynskiy, John C. Haugner III, Aleardo Morelli, Dana Morrone, Burckhard Seelig
    Pages 73-92
  7. Amrita Singh-Blom, Randall A. Hughes, Andrew D. Ellington
    Pages 93-114
  8. Megan F. Cole, Vanessa E. Cox, Kelsey L. Gratton, Eric A. Gaucher
    Pages 115-125
  9. Romualdas Vaisvila, Jurate Bitinaite
    Pages 165-171
  10. Pei-Chung Hsieh, Romualdas Vaisvila
    Pages 173-186
  11. Nili Ostrov, Laura M. Wingler, Virginia W. Cornish
    Pages 187-203
  12. Sandeep Chakraborty, Renu Minda, Lipika Salaye, Abhaya M. Dandekar, Swapan K. Bhattacharjee, Basuthkar J. Rao
    Pages 205-216
  13. Bradley J. Stevenson, Sylvia H.-C. Yip, David L. Ollis
    Pages 237-249
  14. Back Matter
    Pages 251-252

About this book


Whether the pursuit is commercially motivated or purely academic, engineering a novel biological catalyst is an enticing challenge. High-resolution protein structure analysis allows for rational alteration of enzyme function, yet many useful enzyme variants are the product of well-designed selection schemes or screening strategies. Enzyme Engineering: Methods and Protocols provides guidance to investigators wishing to create enzyme variants with desired properties. This detailed volume covers such topics as a simple method for generating site-specific mutations within bacterial chromosomes. It also highlights the engineering of two difference types of rare-cutting endonucleases that show great potential in gene therapy applications: The newest development is the emergence of TAL effector nucleases or TALENs. Chapters describe newly developed technologies in sufficient detail so that each method can be practiced in a standard molecular biology laboratory. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.


Authoritative and easily accessible Enzyme Engineering: Methods and Protocols will be valuable for scientists with a budding interest in protein engineering as well as veterans looking for new approaches to apply in established discovery programs.


bacterial chromosomes biological catalyst enzyme function gene therapy protein engineering

Editors and affiliations

  • James C. Samuelson
    • 1
  1. 1.New England Biolabs, Inc.IpswichUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2013
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-62703-292-6
  • Online ISBN 978-1-62703-293-3
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site