Immune Homeostasis

Methods and Protocols

  • Andrew L. Snow
  • Michael J. Lenardo

Part of the Methods in Molecular Biology book series (MIMB, volume 979)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Min Chen, Lily Huang, Jin Wang
    Pages 51-63
  3. Francis Ka-Ming Chan, Kenta Moriwaki, María José De Rosa
    Pages 65-70
  4. Christopher E. Martin, Kwesi Frimpong-Boateng, Darina S. Spasova, John C. Stone, Charles D. Surh
    Pages 81-106
  5. Liset Westera, Yan Zhang, Kiki Tesselaar, José A. M. Borghans, Derek C. Macallan
    Pages 107-131
  6. Menno C. van Zelm, Magdalena A. Berkowska, Mirjam van der Burg, Jacques J. M. van Dongen
    Pages 133-145
  7. Heather E. Lynch, Gregory D. Sempowski
    Pages 147-159
  8. R. Anthony Barnitz, Sabrina Imam, Kathleen Yates, W. Nicholas Haining
    Pages 161-173
  9. Rebecca A. Marsh, Jack J. Bleesing, Alexandra H. Filipovich
    Pages 189-197
  10. Back Matter
    Pages 213-215

About this book


Many questions remain unresolved as researchers continue to characterize and define the nature of normal immune homeostasis and determine how these processes are dysregulated in immunodeficiency, as well as in autoimmune and lymphoproliferative disorders.  Immune Homeostasis: Methods and Protocols focuses on experimental techniques for measuring and analyzing immune cell dynamics, with a particular emphasis on examining lymphocyte programmed cell death in different contexts.  With contributions by leading experts in the field, the collection examines detailed protocols for studying various pathways of apoptosis and necrosis in different types of hematopoietic cells, both in vitro and in vivo, methods for studying the maintenance of lymphocyte populations in the steady-state or following infectious challenges in both mice and humans, and technical insights into state-of-the-art genomics tools, among other topics.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Valuable and easy to use, Immune Homeostasis: Methods and Protocols serves as an important toolkit for basic and clinical scientists interested in examining various aspects of immune homeostasis in both normal and disease-related contexts in order to further study the dynamic processes that contribute to homeostasis of the immune system.


Cell death or apoptosis Hematopoietic cells Immune cell dynamics Immune homeostasis Immune response Infectious diseases Lymphocytes

Editors and affiliations

  • Andrew L. Snow
    • 1
  • Michael J. Lenardo
    • 2
  1. 1.of the Health Sciences, Department of PharmacologyUniformed Services UniversityBethesdaUSA
  2. 2.NIAID, Laboratory of ImmunologyNational Institutes of HealthBethesdaUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2013
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-62703-289-6
  • Online ISBN 978-1-62703-290-2
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site