Gene Regulation

Methods and Protocols

  • Minou Bina

Part of the Methods in Molecular Biology book series (MIMB, volume 977)

Table of contents

  1. Front Matter
    Pages i-xv
  2. Minou Bina
    Pages 1-11
  3. Matthew Murtha, Yatong Wang, Claudio Basilico, Lisa Dailey
    Pages 35-51
  4. M. van den Boogaard, L. Y. E. Wong, V. M. Christoffels, P. Barnett
    Pages 53-64
  5. Yong Zhong Xu, Cynthia Kanagaratham, Sylwia Jancik, Danuta Radzioch
    Pages 79-93
  6. Cornelia G. Spruijt, H. Irem Baymaz, Michiel Vermeulen
    Pages 137-157
  7. Rebecca H. Blair, James A. Goodrich, Jennifer F. Kugel
    Pages 203-215

About this book

Introduction

In this volume of Methods in Molecular Biology™, expert investigators offer comprehensive, complementary, and cutting-edge technologies for studies of gene regulation.  The chapters of Gene Regulation: Methods and Protocols are organized to provide an integrated and a coherent view of control systems and their associated components.  The protocols are broad in their scope.  They include molecular, biochemical, spectroscopic techniques as well as high throughput strategies.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Comprehensive and broad in their scope, the protocols are useful to researchers in many disciplines including molecular biology, genomics, biochemistry, biomedicine, nutrition, and agricultural sciences.    

FEATURES
• Techniques for studying chromatin structural features that influence gene activation
In vivo and in vitro assays
• Spectroscopic techniques
• Technologies for examining genome-wide association of transcription factors with regulatory regions of genes
• Proteomics based on SILAC (Stable Isotope Labeling of Amino Acids in Cell Culture)
• Peptide microarray technology for studies of kinases
• Schemes for isolation and characterization of relatively large multiprotein complexes
• Biochemical and biophysical techniques for examining gene repression
In vitro chromatin reconstitution to investigate multicomponent transcriptional complexes
• Nuclear recruitment assay to validate transcription factor interactions in mammalian cells

Keywords

DNA sequencing DNase hypersensitivity analysis Heavy methyl-SILAC de novo identification gene activation gene repression histone methylation patterns histone modifications mass spectrometry (LC-MS) protein networks protein-DNA interactions quantitative liquid chromatography

Editors and affiliations

  • Minou Bina
    • 1
  1. 1., Department of ChemistryPurdue UniversityWest LafayetteUSA

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-62703-284-1
  • Copyright Information Springer Science+Business Media, LLC 2013
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-62703-283-4
  • Online ISBN 978-1-62703-284-1
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book