Virus-Induced Gene Silencing

Methods and Protocols

  • Annette Becker

Part of the Methods in Molecular Biology book series (MIMB, volume 975)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Matthias Lange, Aravinda L. Yellina, Svetlana Orashakova, Annette Becker
    Pages 1-14
  3. Arunima Purkayastha, Shweta Sharma, Indranil Dasgupta
    Pages 33-45
  4. Koen Geuten, Tom Viaene, Dries Vekemans, Sofia Kourmpetli, Sinead Drea
    Pages 61-69
  5. Ivan Galis, Meredith C. Schuman, Klaus Gase, Christian Hettenhausen, Markus Hartl, Son T. Dinh et al.
    Pages 109-137
  6. Ben Spitzer-Rimon, Alon Cna’ani, Alexander Vainstein
    Pages 139-148
  7. Chunquan Zhang, Steven A. Whitham, John H. Hill
    Pages 149-156
  8. Josefina-Patricia Fernandez-Moreno, Diego Orzaez, Antonio Granell
    Pages 183-196
  9. Haifeng Jia, Yuanyue Shen
    Pages 211-218
  10. Back Matter
    Pages 219-221

About this book

Introduction

Plants are amazing organisms to study, some are important sources for pharmaceuticals, and others can help to elucidate molecular mechanisms required for a plant’s development and its interactions with the biotic or abiotic environment. Functional genomics is vastly lagging behind the speed of genome sequencing as high-throughput gene function assays are difficult to design, specifically for non-model plants. Bioinformatics tools are useful for gene identification and annotation but are of limited value for predictions concerning gene functions as gene functions are uncovered best by experimental approaches. Virus-Induced-Gene-Silencing (VIGS) is an easy to use, fast, and reliable method to achieve down regulation of target gene expression. Virus-Induced Gene Silencing: Methods and Protocols provides detailed protocols for VIGS experiments in several plant species including model and non-model plants. Also included in this book are recently developed protocols for VIGS-derived microRNA production in the plant or protein over expression, as well as chapters devoted to summarizing the molecular mechanisms of VIGS action and the vector systems developed so far. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

 

Authoritative and easily accessible, Virus-Induced Gene Silencing: Methods and Protocols serves as a valuable resource for researchers from diverse fields of plant biology interested in experimental approaches to analyzing gene functions.

Keywords

VIGS developmental biology genome sequencing phenotypes plant pathogens plant research

Editors and affiliations

  • Annette Becker
    • 1
  1. 1.Institute of BotanyJustus-Liebig-University GießenGießenGermany

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-62703-278-0
  • Copyright Information Springer Science+Business Media New York 2013
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-62703-277-3
  • Online ISBN 978-1-62703-278-0
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book