Lipid-Protein Interactions

Methods and Protocols

  • Jörg H. Kleinschmidt

Part of the Methods in Molecular Biology book series (MIMB, volume 974)

Table of contents

  1. Front Matter
    Pages i-x
  2. Olga Cañadas, Cristina Casals
    Pages 55-71
  3. Luke A. Clifton, Cameron Neylon, Jeremy H. Lakey
    Pages 119-150
  4. Luís M. S. Loura, Manuel Prieto, Fábio Fernandes
    Pages 219-232
  5. Kumud Raj Poudel, Jeffrey P. Jones, James A. Brozik
    Pages 233-252
  6. Christopher Aisenbrey, Matthias Michalek, Evgeniy S. Salnikov, Burkhard Bechinger
    Pages 357-387
  7. Mary A. Schuler, Ilia G. Denisov, Stephen G. Sligar
    Pages 415-433
  8. Teresa Paramo, Diana Garzón, Daniel A. Holdbrook, Syma Khalid, Peter J. Bond
    Pages 435-455
  9. Back Matter
    Pages 457-464

About this book


Biological membranes are the essential structuring elements of all living cells. Many enzymatic reactions take place at the membrane-water interface. To gain detailed insight into membrane properties, it is therefore of great importance to understand the complex nature of the interactions of membrane proteins with lipids. Lipid-Protein Interactions: Methods and Protocols provides a selection of protocols to examine protein-lipid interactions, membrane and membrane protein structure, how membrane proteins affect lipids and how they are in turn affected by the lipid bilayer and lipid properties. The methods described here are all actively used, complementary, and necessary to obtain comprehensive information about membrane structure and function. They include label-free approaches, imaging techniques and spectroscopic methodologies. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, Lipid-Protein Interactions: Methods and Protocols seeks to serve both professional and novices with its wide range of the methods frequently used in this area of research.


NMR spectroscopy lipids membrane membrane protein structure protein-lipid interactions site-directed mutagenesis

Editors and affiliations

  • Jörg H. Kleinschmidt
    • 1
  1. 1.Institut für Biologie, Abteilung BiophysikUniversität KasselKasselGermany

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2013
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-62703-274-2
  • Online ISBN 978-1-62703-275-9
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site