Methods and Protocols

  • Nadine Kabbani

Part of the Methods in Molecular Biology book series (MIMB, volume 964)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Biochemical, Proteomic, and Computational Tools

    1. Front Matter
      Pages 1-1
    2. Jiping Xiao, Clare Bergson
      Pages 3-13
    3. Peiying Yu, Van Anthony Villar, Pedro A. Jose
      Pages 15-24
    4. Ana Lucia Marques Ventura, Fernando Garcia de Mello, Ricardo Augusto de Melo Reis
      Pages 25-42
    5. Kim T. Blackwell, Lane J. Wallace, BoHung Kim, Rodrigo F. Oliveira, Wonryull Koh
      Pages 61-75
  3. Cellular Imaging

    1. Front Matter
      Pages 77-77
    2. Sylwia Lukasiewicz, Agata Faron-Górecka, Marta Dziedzicka-Wasylewska
      Pages 79-94
    3. Gemma Navarro, Peter J. McCormick, Josefa Mallol, Carme Lluís, Rafael Franco, Antoni Cortés et al.
      Pages 95-105
    4. Stefano Espinoza, Bernard Masri, Ali Salahpour, Raul R. Gainetdinov
      Pages 107-122
    5. Wen-Liang Zhou, Katerina D. Oikonomou, Shaina M. Short, Srdjan D. Antic
      Pages 123-138
  4. Genetic Manipulation in Cells and Organisms

  5. Electrochemical, Physiological, and Behavioral Analysis

    1. Front Matter
      Pages 227-227
    2. Shalini Padmanabhan, Thach Pham, Balakrishna M. Prasad
      Pages 229-242
    3. James G. Roberts, Leyda Z. Lugo-Morales, Philip L. Loziuk, Leslie A. Sombers
      Pages 275-294
    4. Anna R. Carta, Ezio Carboni, Saturnino Spiga
      Pages 295-308
  6. Back Matter
    Pages 309-312

About this book


Dopamine, a catecholamine transmitter, plays a number of vital physiological roles in the brain and body, and, in recent years, studies on the role of dopamine in disease have opened new avenues of research and discovery.  In Dopamine: Methods and Protocols, experts and key figures within the field provide detailed protocols on leading approaches in the study of dopamine within biological systems. Divided into sections on cellular/biochemical, imaging, genetics, and electrophysiology, this collections includes protocols for bioluminescence and fluorescence imaging, receptor immunoprecipitation and proteomic analysis, creation and characterization of a mouse model of Parkinson’s disease, real time measurement of dopamine in the brain, and modeling signal transduction in silico.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective subjects, lists of materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Authoritative and convenient, Dopamine: Methods and Protocols serves as an ideal guide for students and experts alike, as well as for anyone interested in exploring the vast and crucial field of dopamine research.


Catecholamine Cellular imaging Dopamine Electrophysiology Genetic manipulation Neurotransmitter Physiological role

Editors and affiliations

  • Nadine Kabbani
    • 1
  1. 1.Krasnow Institute for Advanced Study, Dept. of Molecular NeuroscienceGeorge Mason UniversityFairfaxUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2013
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-62703-250-6
  • Online ISBN 978-1-62703-251-3
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site