Bacterial Cell Surfaces

Methods and Protocols

  • Anne H. Delcour

Part of the Methods in Molecular Biology book series (MIMB, volume 966)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Yizhou Zhou, Daniel R. Smith, David A. Hufnagel, Matthew R. Chapman
    Pages 53-75
  3. Chungyu Chang, I-Hsiu Huang, Antoni P. A. Hendrickx, Hung Ton-That
    Pages 77-95
  4. Adrien Ducret, Olivier Théodoly, Tâm Mignot
    Pages 97-107
  5. Susan M. Steenbergen, Eric R. Vimr
    Pages 109-120
  6. Elena B. Tikhonova, Helen I. Zgurskaya
    Pages 133-155
  7. Tanya L. Johnson, Aleksandra E. Sikora, Ryszard A. Zielke, Maria Sandkvist
    Pages 157-172
  8. Eric Faudry, Caroline Perdu, Ina Attrée
    Pages 173-185
  9. Mayukh K. Sarkar, Seyyed I. Husnain, Simon J. Jakubowski, Peter J. Christie
    Pages 187-204
  10. Jan Grijpstra, Martine P. Bos, Jan Tommassen
    Pages 223-237
  11. Jessica V. Hankins, James A. Madsen, Brittany D. Needham, Jennifer S. Brodbelt, M. Stephen Trent
    Pages 239-258
  12. Halima Chutkan, Ian MacDonald, Andrew Manning, Meta J. Kuehn
    Pages 259-272
  13. Jacob Biboy, Nhat Khai Bui, Waldemar Vollmer
    Pages 273-288
  14. Filipe Carvalho, María Graciela Pucciarelli, Francisco García-del Portillo, Didier Cabanes, Pascale Cossart
    Pages 289-308
  15. Katleen Denoncin, Valérie Nicolaes, Seung-Hyun Cho, Pauline Leverrier, Jean-François Collet
    Pages 325-336
  16. Shu Quan, Annie Hiniker, Jean-François Collet, James C. A. Bardwell
    Pages 359-366
  17. Boris Martinac, Paul R. Rohde, Charles G. Cranfield, Takeshi Nomura
    Pages 367-380
  18. Back Matter
    Pages 397-399

About this book


In recent years, molecular microbiology has emerged as a top, cutting-edge biological discipline, thanks to the multi-disciplinary and integrative approaches taken by investigators seeking to understand the intricacies of the microbial world and how it affects human health and the biosphere. In Bacterial Cell Surfaces: Methods and Protocols, recent advances in structural biology, proteomics, and imaging techniques, together with the traditional biochemical and genetic approaches, are provided in order to present an exciting look into the structure, function, and regulation of the bacterial cell envelope. The detailed volume contains examples of traditional and innovative tools for the study of protein structure and function and enzymatic activities, the purification and analysis of macromolecules and their complexes, and the investigation of regulatory mechanisms and cell biological processes.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Authoritative and practical, Bacterial Cell Surfaces: Methods and Protocols is aimed at the microbiologist, biochemist, or cell biologist, whether a beginning graduate student or a veteran experimentalist, who wishes to learn new methodologies and take advantage of the years of research and protocol optimization from the best laboratories around the world.


Bacterial envelope Bacterium Enzymatic activities Gram-negative bacteria Macromolecule purification, analysis Molecular microbiology Mycobacteria Protein structure, function Regulatory mechanisms The bacterial cell surface

Editors and affiliations

  • Anne H. Delcour
    • 1
  1. 1., Dept. of Biology and BiochemistryUniversity of HoustonHoustonUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2013
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-62703-244-5
  • Online ISBN 978-1-62703-245-2
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site