JAK-STAT Signalling

Methods and Protocols

  • Sandra E. Nicholson
  • Nicos A. Nicola

Part of the Methods in Molecular Biology book series (MIMB, volume 967)

Table of contents

  1. Front Matter
    Pages i-xi
  2. JAK Protein Tyrosine Kinases

    1. Front Matter
      Pages 1-1
    2. Jeremy A. Ross, Georgialina Rodriguez, Robert A. Kirken
      Pages 3-20
    3. Jeffrey J. Babon, James M. Murphy
      Pages 39-55
    4. Katherine H. Fisher, Stephen Brown, Martin P. Zeidler
      Pages 81-97
    5. Christopher J. Burns, David Segal, Andrew F. Wilks
      Pages 99-113
    6. Anthony J. Bench, E. Joanna Baxter, Anthony R. Green
      Pages 115-131
  3. Signal Transducers and Activators of Transcription

    1. Front Matter
      Pages 133-133
    2. Jean-Patrick Parisien, Curt M. Horvath
      Pages 135-145
    3. Jane Murphy, Gabrielle L. Goldberg
      Pages 161-165
    4. Torsten Ginter, Thorsten Heinzel, Oliver H. Krämer
      Pages 167-178
    5. Velasco Cimica, Nancy C. Reich
      Pages 189-202
    6. Lynn M. Heltemes-Harris, Michael A. Farrar
      Pages 225-232
  4. Suppressor of Cytokine Signalling Proteins

    1. Front Matter
      Pages 233-233
    2. Jeffrey J. Babon, Artem Laktyushin, Nadia J. Kershaw
      Pages 261-271
  5. Structural Analysis of the JAK/STAT Pathway

    1. Front Matter
      Pages 273-273
    2. Isabelle S. Lucet, Rebecca Bamert
      Pages 275-300
  6. Back Matter
    Pages 319-320

About this book


The receptor-associated JAK protein kinases and their substrates, the STAT transcriptional activators, transmit signals following cytokine and growth factor binding to receptors expressed on the cell surface, to result in specific transcriptional and cellular responses. Over the last two decades, the field has progressed from identification of the individual components through to an understanding of the activation and deactivation mechanisms, and the complex structural detail of the proteins involved. We now know that these pathways are important in many biological processes, including growth and development, hematopoiesis, and the innate and adaptive immune response. JAK-STAT Signalling: Methods and Protocols provides detailed methodologies for examining many aspects of the pathway. Divided into four sections, topics include JAK and STAT specific approaches, the negative regulators of the pathway (SOCS proteins), and the production and crystallization of JAK and STAT proteins, among others. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.


Authoritative and easily accessible, JAK-STAT Signalling: Methods and Protocols will be of use not only to those working in the area but also to new investigators who are led to delve into the complexities of JAK and STAT responses.


JAK protein kinases SOCS proteins STAT proteins STAT transcriptional activators tyrosine proteins

Editors and affiliations

  • Sandra E. Nicholson
    • 1
  • Nicos A. Nicola
    • 2
  1. 1.Walter and Eliza Hall InstituteParkvilleAustralia
  2. 2.Walter and Eliza Hall InstituteParkvilleAustralia

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2013
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-62703-241-4
  • Online ISBN 978-1-62703-242-1
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site