Cell Senescence

Methods and Protocols

  • Lorenzo Galluzzi
  • Ilio Vitale
  • Oliver Kepp
  • Guido Kroemer

Part of the Methods in Molecular Biology book series (MIMB, volume 965)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Andrew R. J. Young, Masako Narita, Masashi Narita
    Pages 1-13
  3. Vjekoslav Dulic
    Pages 15-35
  4. Yingjuan Qian, Xinbin Chen
    Pages 37-61
  5. Amancio Carnero
    Pages 63-81
  6. Hong Zhao, Zbigniew Darzynkiewicz
    Pages 83-92
  7. Ilio Vitale, Mohamed Jemaà, Lorenzo Galluzzi, Didier Metivier, Maria Castedo, Guido Kroemer
    Pages 93-120
  8. Oliver Kepp, Isabelle Martins, Laurie Menger, Mickaël Michaud, Sandy Adjemian, Abdul Qader Sukkurwala et al.
    Pages 121-142
  9. Koji Itahana, Yoko Itahana, Goberdhan P. Dimri
    Pages 143-156
  10. Vinicius Bassaneze, Ayumi Aurea Miyakawa, José Eduardo Krieger
    Pages 157-163
  11. Katherine M. Aird, Rugang Zhang
    Pages 185-196
  12. Glyn Nelson, Thomas von Zglinicki
    Pages 197-213
  13. Michelle F. Maritz, Laura A. Richards, Karen L. MacKenzie
    Pages 215-231
  14. Asako J. Nakamura
    Pages 233-242
  15. Clea Bárcena, Fernando G. Osorio, José Maria Pérez Freije
    Pages 243-251
  16. João F. Passos, Satomi Miwa, Thomas von Zglinicki
    Pages 253-263
  17. Nancy B. Wehr, Rodney L. Levine
    Pages 265-281
  18. Ana Cipak Gasparovic, Morana Jaganjac, Branka Mihaljevic, Suzana Borovic Sunjic, Neven Zarkovic
    Pages 283-296
  19. J. Renwick Beattie, John J. McGarvey, Alan W. Stitt
    Pages 297-312
  20. Sieu L. Tran, Helen Rizos
    Pages 313-326
  21. Sofia Chiatamone Ranieri, Giovambattista Pani
    Pages 341-353
  22. Florian M. Geier, Silke Fuchs, Gabriel Valbuena, Armand M. Leroi, Jacob G. Bundy
    Pages 355-371
  23. Narendra Wajapeyee, Sara K. Deibler, Michael R. Green
    Pages 373-382
  24. Tatiana V. Pospelova, Zhanna V. Chitikova, Valery A. Pospelov
    Pages 383-408
  25. Clara Correia-Melo, Diana Jurk, João F. Passos
    Pages 409-419
  26. Stavros Gonidakis, Valter D. Longo
    Pages 421-437
  27. Heinz D. Osiewacz, Andrea Hamann, Sandra Zintel
    Pages 439-462
  28. Jia Hu, Min Wei, Mario G. Mirisola, Valter D. Longo
    Pages 463-472
  29. Reza Ardekani, Simon Tavaré, John Tower
    Pages 501-516
  30. Sandra Donnini, Antonio Giachetti, Marina Ziche
    Pages 517-531
  31. Back Matter
    Pages 533-538

About this book


Cell senescence is the process whereby cells permanently lose the possibility to proliferate without undergoing cell death, and occurs in a plethora of distinct model organisms. In Cell Senescence: Methods and Protocols, expert researchers in the field detail the methods that are now commonly used to study cell senescence, in model organisms encompassing bacteria, fungi, worms, flies, zebrafish, and mammalian cells. These techniques cover the study of all the morphological, biochemical and functional manifestations of senescence at the cellular level and include protocols for population analyses and high-throughput approaches in suitable model organisms. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.


autophagy biochemical manifestations cell cycle-arresting proteins cell death chemokyne signaling functional manifestations heterochromatin foci mammalian cells model organisms morphological features secretory phenotype

Editors and affiliations

  • Lorenzo Galluzzi
    • 1
  • Ilio Vitale
    • 2
  • Oliver Kepp
    • 3
  • Guido Kroemer
    • 4
  1. 1.Institut Gustave Roussy, Pavillon de Recherche 1INSERM U848VillejuifFrance
  2. 2.Institut Gustave Roussy, Pavillon de Recherche 1INSERM U848Villejuif CEDEXFrance
  3. 3.Institut Gustave Roussy, Pavillon de Recherche 1INSERM U848Villejuif CEDEXFrance
  4. 4.Institut Gustave Roussy (IGR), Pavillon de Recherche 1INSERM, U848VillejuifFrance

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2013
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-62703-238-4
  • Online ISBN 978-1-62703-239-1
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site