Plant Organogenesis

Methods and Protocols

  • Ive De Smet

Part of the Methods in Molecular Biology book series (MIMB, volume 959)

Table of contents

  1. Front Matter
    Pages i-xvi
  2. Yogev Burko, Naomi Ori
    Pages 1-19
  3. Sandrine Bonhomme, Fabien Nogué, Catherine Rameau, Didier G. Schaefer
    Pages 21-43
  4. Julien Lavenus, Mikaël Lucas, Laurent Laplaze, Soazig Guyomarc’h
    Pages 45-67
  5. Caroline Marcon, Anja Paschold, Frank Hochholdinger
    Pages 69-81
  6. Kenny A. Bogaert, Alok Arun, Susana M. Coelho, Olivier De Clerck
    Pages 97-125
  7. Balaji Enugutti, Maxi Oelschner, Kay Schneitz
    Pages 127-135
  8. Cristina Llavata-Peris, Annemarie Lokerse, Barbara Möller, Bert De Rybel, Dolf Weijers
    Pages 137-148
  9. Leen Jansen, Boris Parizot, Tom Beeckman
    Pages 149-158
  10. Anjali S. Iyer-Pascuzzi, Paul R. Zurek, Philip N. Benfey
    Pages 177-187
  11. Dominique Audenaert, Bert De Rybel, Long Nguyen, Tom Beeckman
    Pages 189-195
  12. John Runions, Smita Kurup
    Pages 197-205
  13. Cristian Forestan, Nicola Carraro, Serena Varotto
    Pages 207-222
  14. Cristian Forestan, Serena Varotto
    Pages 223-233
  15. Ram Kishor Yadav, Stephen Snipes, Thomas Girke, G. Venugopala Reddy
    Pages 235-245
  16. Hilde Nelissen, Bart Rymen, Frederik Coppens, Stijn Dhondt, Fabio Fiorani, Gerrit T. S. Beemster
    Pages 247-264
  17. Kaoru Sugimoto, Elliot M. Meyerowitz
    Pages 265-275
  18. Angelika Mustroph, M. Eugenia Zanetti, Thomas Girke, Julia Bailey-Serres
    Pages 277-302
  19. Pilar Bustos-Sanmamed, Carole Laffont, Florian Frugier, Christine Lelandais-Brière, Martin Crespi
    Pages 303-316
  20. Pilar Bustos-Sanmamed, Carole Laffont, Florian Frugier, Christine Lelandais-Brière, Martin Crespi
    Pages 317-322
  21. Roeland M. H. Merks, Michael A. Guravage
    Pages 333-352
  22. Back Matter
    Pages 353-356

About this book


Organogenesis entails the regulation of cell division, cell expansion, cell and tissue type differentiation, and patterning of the organ as a whole. It is essential to gain insight into how organs are initiated and how they develop. However, this very often is subject to technical difficulties as these processes take place embedded deep in tissues or are difficult to access or visualize. To achieve this, we need specialized techniques such as those concisely illustrated in Plant Organogenesis: Methods and Protocols. Chapters address topics such as how to study and image the structure of ovules and embryos of Arabidopsis thaliana, tools to establish cell lineages in order to visualize the contribution of each cell and cell division to the building of a mature organ, approaches to study the totipotency of several plant cells, techniques such as the use of fluorescence-activated cell sorting (FACS) to analyse transcriptomes and hormone levels in Arabidopsis, methods to investigate organogenesis in economically important crops, and computer-based approaches to bring everything together. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.


Authoritative and easily accessible, Plant Organogenesis: Methods and Protocols serves both professionals and novices with its well-honed methodologies in an effort to further our knowledge of this fascinating research field.


cell lineage analysis de novo organogenesis model systems ovule development plant organogenesis root systems tissue culture

Editors and affiliations

  • Ive De Smet
    • 1
  1. 1.School of BiosciencesUniversity of NottinghamLoughboroughUnited Kingdom

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2013
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-62703-220-9
  • Online ISBN 978-1-62703-221-6
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site