Rice Protocols

  • Yinong Yang

Part of the Methods in Molecular Biology book series (MIMB, volume 956)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Jason G. Walling, Wenli Zhang, Jiming Jiang
    Pages 13-27
  3. Jennifer L. Cooper, Steven Henikoff, Luca Comai, Bradley J. Till
    Pages 39-56
  4. Vibha Srivastava
    Pages 83-93
  5. Guru Jagadeeswaran, Ramanjulu Sunkar
    Pages 109-118
  6. Norman Warthmann, Stephan Ossowski, Rebecca Schwab, Detlef Weigel
    Pages 131-149
  7. Ganesh Kumar Agrawal, Nam-Soo Jwa, Young-Ho Jung, Sun Tae Kim, Dea-Wook Kim, Kyoungwon Cho et al.
    Pages 151-184
  8. Kyoungwon Cho, Oksoo Han, Shigeru Tamogami, Junko Shibato, Akihiro Kubo, Ganesh Kumar Agrawal et al.
    Pages 185-200
  9. Nan Zhao, Xiaofeng Zhuang, Gitika Shrivastava, Feng Chen
    Pages 201-208
  10. Utlwang Batlang, Niranjan Baisakh, Madana M. R. Ambavaram, Andy Pereira
    Pages 209-225
  11. Benildo G. de los Reyes, Song Joong Yun, Venura Herath, Fuyu Xu, Myoung Ryoul Park, Jeong Il Lee et al.
    Pages 227-241
  12. Zhiming Yu, Hanmin Chen, Yanli Tong, Ping Wu
    Pages 243-248
  13. Yulin Jia, Guangjie Liu, Dong-Soo Park, Yinong Yang
    Pages 257-268
  14. Junya Mizoi, Kazuko Yamaguchi-Shinozaki
    Pages 269-283
  15. Emily E. Helliwell, Yinong Yang
    Pages 285-309
  16. Back Matter
    Pages 327-335

About this book


With the completion of a finished rice genome sequence, increasing efforts have focused on functional characterization of rice genes, elucidation of the underlying mechanisms involved in major agronomic traits (e.g., high yield, grain quality, abiotic stress tolerance, and disease resistance), and the subsequent translation of genomic knowledge into agricultural productivity via molecular breeding and improved cultural practice. To meet increasing interest in this field, Rice Protocols has been compiled to provide a series of core techniques and approaches commonly used in studying rice molecular biology and functional genomics. These approaches include genetic and molecular techniques such as artificial hybridization, fluorescence in situ hybridization, generation and characterization of chemical and T-DNA insertional mutants, quantitative trait loci (QTLs) analysis and map-based cloning, site-specific transgene integration, and artificial microRNA-mediated gene silencing, along with a variety of “omics” techniques.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Authoritative and easy to use, Rice Protocols will prove useful for both beginners and experienced researchers whether they are molecular biologists who want to study rice plants or rice researchers who are interested in learning molecular techniques.


Agriculture Cereal crops Functional genomics Molecular breeding Oryza sativa Rice genes Rice molecular biology

Editors and affiliations

  • Yinong Yang
    • 1
  1. 1.Huck Institutes of Life Sciences, Department of Plant PathologyPennsylvania State UniversityUniversity ParkUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2013
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-62703-193-6
  • Online ISBN 978-1-62703-194-3
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site